Allicin alleviated learning and memory deficits caused by lead exposure at developmental stage
Allicin alleviated learning and memory deficits caused by lead exposure at developmental stage
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大蒜素可缓解发育阶段铅暴露引起的学习和记忆缺陷
DOI：
10.1016/j.lfs.2019.06.007
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发表时间：
2019-08-15
期刊：
影响因子：
6.1
通讯作者：
Zhao, Lina
中科院分区：
文献类型：
--
作者：
Cai, Shizhong;Liu, Jun;Zhao, Lina
目的：探索铅中毒的治疗策略是一条很有前途的途径。大蒜素是从大蒜中提取的一种天然化合物，具有许多有益的生物学特性。本研究旨在探讨大蒜素对发育期醋酸铅染毒大鼠学习记忆功能的保护作用。材料与方法：采用醋酸铅诱导大鼠学习记忆功能损伤，并灌胃大蒜素以减轻其毒性。Morris水迷宫实验检测大鼠学习记忆功能。测定超氧化物歧化酶（SOD）、谷胱甘肽（GSH）和丙二醛（MDA）的含量，以确定氧化应激。进行免疫荧光以分析GFAP阳性细胞。结果：大蒜素可通过促进海马星形胶质细胞的分化来改善醋酸铅所致的学习记忆障碍，这主要是通过EGFR/ERK信号通路实现的。此外，大蒜素衰减增加的ROS水平通过调节氧化防御system.Significance：这些结果表明，大蒜素是一种有效的代理能够改善醋酸铅诱导的学习和记忆缺陷在早期发展，因此可能是有用的击败醋酸铅毒性。
Aims: It is a promising approach to search the therapeutic strategies for treating lead (Pb) toxicity. Allicin, a natural compound extracted from Allium sativum (garlic), has been reported to have many beneficially biological properties. In this study, we investigated the protective effects of allicin on learning and memory function of rats exposed by lead acetate at developmental stage.Materials and methods: Rats received lead acetate for inducing toxicity, and gavaged with allicin to ameliorate this toxicity. Morris water maze test was performed to determine learning and memory function. Superoxide dismutase (SOD), glutathione (GSH) and methane dicarboxylic aldehyde (MDA) was measured to determine oxidative stress. Immunofluorescence was carried out to analyze GFAP-positive cells. The protein expression of ERK, p-ERK, EGFR and p-EGFR were detected using western blot.Key findings: We found that allicin ameliorated lead acetate-caused learning and memory deficits by promoting hippocampus astrocyte differentiation, which mainly through EGFR/ERK signaling. Moreover, allicin attenuated the increased ROS level by regulating the oxidative defense system.Significance: These results suggest that allicin is a potent agent able to ameliorate lead acetate-induced learning and memory deficits during early development, and may thus be useful for defeating lead acetate toxicity.