High occurrence rate of tetracycline (TC)-resistant bacteria and TC resistance genes relates to microbial diversity in sediment of Mekong River main waterway
High occurrence rate of tetracycline (TC)-resistant bacteria and TC resistance genes relates to microbial diversity in sediment of Mekong River main waterway
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DOI：
10.1264/jsme2.23.149
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发表时间：
2008-01-01
影响因子：
2.2
通讯作者：
Tana, Touch Seang
中科院分区：
文献类型：
--
作者：
Suzuki, Satoru;Kobayashi, Takeshi;Tana, Touch Seang
对湄公河流域底泥中四环素(TC)耐药细菌的空间监测表明，主要航道的TC耐药细菌的出现率较高，而洞里萨湖和西贡河口的四环素抗性细菌的出现率较低。利用变性梯度凝胶电泳法(DGGE)计算了指示生态多样性的香农指数(H‘)，表明湄公河主航道的微生物多样性高于洞里萨湖和西贡河口，这种多样性与抗TC细菌的出现率呈正相关。对同一地区核糖体保护蛋白基因tet(M)、tet(S)和tet(W)的分析也表明，rpp基因的高度多样性与rpp基因的出现率呈正相关，表明rpp基因在不同细菌物种中具有很好的保守性。不同基因类型tet(M)的进一步证据表明，耐药基因可能有不同的来源，并在沉积物中混合。因此，该地区的沉积物是耐药基因的潜在储存库。
Spatial monitoring of tetracycline (TC)-resistant bacteria in sediments of the Mekong River watershed revealed that the main waterway showed a high occurrence rate of TC-resistant bacteria, whereas Tonle Sap Lake and the Sai Gon estuary did not. The Shannon index (H'), an indicator of ecological diversity, was calculated from denaturing gradient gel electrophoresis (DGGE) profiles, which indicated that the main waterway of the Mekong River had high microbial diversity (high H') compared to Tonle Sap Lake and the Sai Gon estuary; this diversity was positively correlated with the occurrence rate of TC-resistant bacteria. Analysis of ribosomal protection protein (RPP) genes tet(M), tet(S) and tet(W) in the same area also revealed that high diversity was positively correlated with the occurrence rate of RPP genes, suggesting that RPP genes are well conserved across various bacterial species. Further evidence of different genotypes of tet(M) suggests that the drug resistance genes likely have various origins, and are mixed in the sediment. Sediments in this area are therefore potential reservoirs of drug resistance genes.