Identification of C-type natriuretic peptide in heart of spiny dogfish shark (Squalus acanthias).
Identification of C-type natriuretic peptide in heart of spiny dogfish shark (Squalus acanthias).
复制标题
角鲨 (Squalus acanthias) 心脏中 C 型钠尿肽的鉴定。
DOI：
10.1152/ajprenal.1991.261.4.f734
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
ForrestJr,JN
中科院分区：
文献类型：
--
作者：
Schofield,JP;Jones,DS;ForrestJr,JN
一种未鉴定的心房钠尿肽（ANP）样物质是调节鲨鱼直肠腺中氯化钠分泌的主要激素，鲨鱼直肠腺是激素敏感的二次活性氯转运的上皮模型组织。哺乳动物 ANP 抗体不能识别海洋物种的激素原。使用聚合酶链式反应 (PCR) 分离编码鲨鱼心钠尿肽的部分 cDNA。使用该部分序列作为探针，从鲨鱼心脏 cDNA 文库中获得全长克隆 [882 个碱基对 (bp)]。氨基酸 119-135 与最近鉴定的猪 C 型利钠肽 (CNP) 和从这些物种的大脑中分离的鳉鱼脑利钠肽的肽序列相似。与所有其他物种的心脏 ANP 样肽相反，成熟鲨鱼心脏 CNP 在第二个半胱氨酸残基处终止，并且缺乏 COOH 末端延伸。鲨鱼心脏前 CNP 前体的一级氨基酸序列与所有其他心脏钠尿肽明显不同。跨越编码区的基因组 DNA 的扩增产生了 1.5 kb 的产物，表明存在至少一个内含子。测序证实存在两个 90 bp 和 315 bp 的外显子，由 1.1 kb 内含子分隔。这是在非神经元组织中编码 cDNA 的第一份报告。软骨鱼类 CNP 可能代表 ANP 样肽的原始形式，它是为了适应环境渗透压调节应激而进化的。
An unidentified atrial natriuretic peptide (ANP)-like substance is the principal hormone regulating NaCl secretion in the shark rectal gland, an epithelial model tissue for hormone-sensitive secondary active chloride transport. Antibodies to mammalian ANP do not recognize the prohormone of marine species. The polymerase chain reaction (PCR) was used to isolate a partial cDNA encoding the shark heart natriuretic peptide. Using this partial sequence as a probe, the full-length clone [882 base pairs (bp)] was obtained from a shark heart cDNA library. Amino acids 119-135 are similar to the recently identified peptide sequences of porcine C-type natriuretic peptide (CNP) and killifish brain natriuretic peptide isolated from the brain of these species. Mature shark heart CNP terminates at the second cysteine residue and lacks a COOH-terminal extension, in contrast to cardiac ANP-like peptides of all other species. The primary amino acid sequence of the shark heart prepro-CNP is distinctly different from all other cardiac natriuretic peptides. Amplification of genomic DNA spanning the coding region produced a 1.5-kb product, indicating the presence of at least one intron. Sequencing confirmed the presence of two exons of 90 and 315 bp, separated by a 1.1-kb intron. This is the first report of a cDNA encoding in nonneuronal tissue. Elasmobranch CNP may represent a primordial form of ANP-like peptides that evolved as an adaptation to environmental osmoregulatory stress.