Characterization of TLR4 agonist effects on alhydrogel® sedimentation: A novel application of laser scattering optical profiling
Characterization of TLR4 agonist effects on alhydrogel® sedimentation: A novel application of laser scattering optical profiling
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DOI：
10.1002/jps.23307
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发表时间：
2012-11-01
影响因子：
3.8
通讯作者：
Fox, Christopher B.
中科院分区：
文献类型：
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作者：
Fox, Christopher B.
几十年来，铝盐制剂如Alhydrogel（R）已被用作有效和安全的疫苗佐剂。铝盐对疫苗抗原和其他免疫调节分子如Toll样受体（TLR）激动剂具有高吸附能力。然而，一些吸附TLR激动剂的表征具有挑战性，需要破坏性分析、复杂仪器和/或解吸方法开发。在这份报告中，我们使用激光散射光学轮廓表征了吸附了合成Toll样受体4（TLR 4）激动剂的Alhydrogel（R）对铝盐沉降行为的影响。该方法有助于检测少量吸附的TLR 4激动剂对铝盐沉降行为的影响（
Aluminum salt formulations such as Alhydrogel (R) have been used for decades as effective and safe vaccine adjuvants. Aluminum salts have a high adsorptive capacity for vaccine antigens and other immunomodulatory molecules such as Toll-like receptor (TLR) agonists. However, the characterization of some Alhydrogel (R)-adsorbed TLR agonists is challenging, requiring destructive analysis, complex instrumentation, and/or desorption method development. In this report, we characterize the effects of an Alhydrogel (R)-adsorbed synthetic Toll-like receptor 4 (TLR4) agonist on aluminum salt sedimentation behavior using laser scattering optical profiling. This method facilitates the detection of the effects on aluminum salt sedimentation behavior of low amounts of adsorbed TLR4 agonist (