Photometric flow injection determination of phosphate on a PDMS microchip using an optical detection system assembled with an organic light emitting diode and an organic photodiode
Photometric flow injection determination of phosphate on a PDMS microchip using an optical detection system assembled with an organic light emitting diode and an organic photodiode
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DOI：
10.1016/j.talanta.2014.08.057
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发表时间：
2015-01-15
期刊：
影响因子：
6.1
通讯作者：
Imato, Toshihiko
中科院分区：
文献类型：
--
作者：
Liu, Rong;Ishimatsu, Ryoichi;Imato, Toshihiko
以铕配合物europium(diben-zoylmethanato)(3)(bathophenanthroline)(Eu(DBM)(3))为光源，在聚二甲基硅氧烷(PDMS)微芯片上，以铜(CuPc)/富勒烯(C-60)两层异质结有机光电二极管为光电探测器，构建了一种紧凑的光度检测器，并将其应用于磷酸盐的测定。采用气相沉积方法在ITO镀膜玻璃衬底上制备了OLED和OPD。玻璃(0.7 mm)/ITO(110 Nm)/4，4‘-双[N-(1-萘基)-N-苯氨基]联苯(α-NPD)(30 Nm)/4，4’-二(N-咔唑)联苯(CBP)：Eu3+(8wt%，30 nm)/亚铜(BCP)(30 Nm)/铝三(8-羟基喹啉)(Alq(3))(25 Nm)/镁和银(100 Nm)/银(10 Nm)和玻璃(0.7 mm)/ITO(110 Nm)/CuPc(35 Nm)/C-60(50 Nm)/BCP(10 Nm)/Ag(50 Nm)。基于Eu络合物的OLED在612 nm处发射了锐利的光，半高全宽(FWHM)为8 nm。通过测量不同浓度的孔雀石绿(MG)溶液在1 cm长的间歇体系中的吸光度，对组装的光度检测器的性能进行了评估。由OPD的光电流计算得到的MG溶液的摩尔吸收系数与文献报道的值符合得很好。用传统的光刻方法制备了两进一出U形通道的微芯片。OLED和OPD被配置为通过PDMS微芯片平行面对对方，以使OLED和OPD的光轴与位于出口末端的流动池(光路长度为5 mm)对准。利用MG与磷钼络合物的离子缔合反应，在0-0.2ppm浓度范围内，浓度与吸光度呈良好的线性关系，检出限(S/N=3)为0.02ppm。将组装好的光度检测器用于流动注射河流水样，在硫酸中加入MG和钼铵试剂溶液测定磷酸盐。对添加0.08ppm标准浓度的河水样品进行了良好的回收率(97-99%)，相对标准偏差约为5%(n=5)。(C)2014爱思唯尔B.V.保留所有权利。
A compact photometric detector was constructed from an organic light emitting diode (OLED) based on a europium complex, europium(diben-zoylmethanato)(3)(bathophenanthroline) (Eu(DBM)(3)bath), as the light source and an organic photodiode (OPD) fabricated from a hetero-junction of two layers of copper phthalocyanine (CuPc)/fullerene (C-60) as the photo-detector on a microchip prepared from poly (dimethylsiloxan) (PDMS) and was applied to the determination of phosphate. The OLED and the OPD were fabricated by a vapor deposition method on an indium tin oxide (ITO) coated glass substrate with the following layered structure; Glass (0.7 mm)/ITO (110 nm)/4,4'-bis[N-(1-naphthyl)-N-phenyl amino]biphenyl (alpha-NPD) (30 nm)/4,4'-di(N-carbazolyl)biphenyl (CBP): Eu3+ (8 wt%, 30 nm)/bathocuproine (BCP) (30 nm)/aluminum tris(8-hydroxyquinoline) (Alq(3)) (25 nm)/magnesium and silver (MgAg) (100 nm)/Ag (10 nm) and Glass (0.7 mm)/ITO (110 nm)/CuPc (35 nm)/C-60 (50 nm)/BCP (10 nm)/Ag (50 nm), respectively. The OLED based on the europium complex emitted a sharp light at the wavelength of 612 nm with a full width at half maximum (FWHM) of 8 nm. The performance of the photometric detector assembled was evaluated based on measurements of the absorbance of different concentrations of malachite green (MG) solutions for a batch system with 1 cm long path length. The molar absorptive coefficient of the MG solution, calculated from the photocurrent of the OPD, was in good agreement with the value reported in the literature. A microchip with two inlets and one outlet U-shaped channel was prepared by a conventional photolithograph method. The OLED and the OPD were configured so as to face each other through the PDMS microchip in parallel in order to align the light axis of the OLED and the OPD with the flow cell (optical path length of 5 mm), which was located at the end of outlet. For the determination of phosphate, an ion-association reaction between MG and a molybdenum-phosphate complex was utilized and a good linear relationship between the concentration and absorbance was observed in the concentration range 0-0.2 ppm, with a detection limit (S/N=3) of 0.02 ppm. The assembled photometric detector was also applied to the determination of phosphate by the flow injection of river water samples using the reagent solution containing MG and molybdenum ammonium in sulfuric acid. A good recovery (97-99%) for the river water samples, which had been spiked with the standard 0.08 ppm, with an RSD of ca 5% (n=5) was obtained using the constructed system. (C) 2014 Elsevier B.V. All rights reserved.