The short neuropeptide F receptor regulates olfaction-mediated foraging behavior in the oriental fruit fly Bactrocera dorsalis (Hendel)
The short neuropeptide F receptor regulates olfaction-mediated foraging behavior in the oriental fruit fly Bactrocera dorsalis (Hendel)
复制标题
短神经肽 F 受体调节东方果蝇 Bactrocera dorsalis (Hendel) 嗅觉介导的觅食行为
DOI：
10.1016/j.ibmb.2021.103697
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发表时间：
2021-12-08
影响因子：
3.8
通讯作者：
Jiang, Hongbo
中科院分区：
文献类型：
--
作者：
Li, Hongfei;Huang, Xingying;Jiang, Hongbo
短神经肽F（sNPF）信号系统由sNPF及其受体（sNPFR）组成，影响昆虫的摄食、生长和嗅觉记忆等生理过程。我们以前表明，sNPF调节嗅觉灵敏度在东方果蝇桔小实蝇（亨德尔）在饥饿期间。然而，sNPFR的功能分析受到该物种RNA干扰失败的限制。在这里，我们使用CRISPR/Cas9系统生成了一个无效sNPFR突变体，以更详细地研究该受体的生理作用。G 0成虫产生的频率为60.8%，和sNPFR-/-突变体后，获得几代回交，然后自交杂合苍蝇。我们发现，突变体在觅食某些食物时明显不太成功，并表现出觅食潜伏期增加。触角电位（EAG）分析表明，突变体有显着降低的电生理反应，三个测试的气味。此外，qPCR数据揭示了几种嗅觉受体基因的抑制，包括Orco。免疫组化结果显示，BdsNPFR定位于触角感器下的细胞中。基于它们的形状和大小，BdsNPFR+细胞不同于气味受体神经元（ORN），其使用果蝇Orco抗体标记。我们的数据表明，sNPFR调节嗅觉介导的觅食行为，通过介导BdsNPFR+细胞和选定的ORN之间的相互作用。
The short neuropeptide F (sNPF) signaling system, consisting of sNPF and its receptor (sNPFR), influences many physiological processes in insects, including feeding, growth and olfactory memory. We previously showed that sNPF regulates olfactory sensitivity in the oriental fruit fly Bactrocera dorsalis (Hendel) during starvation. However, the functional analysis of sNPFR is constrained by the failure of RNA interference in this species. Here, we generated a null sNPFR mutant using the CRISPR/Cas9 system to investigate the physiological roles of this receptor in more detail. G0 adults were produced at a frequency of 60.8%, and sNPFR-/- mutants were obtained after several generations of backcrossing followed by self-crossing among heterozygous flies. We found that the mutants were significantly less successful at foraging for certain foods and showed increased foraging latency. Electroantennogram (EAG) assays indicated that the mutants had significantly lower electrophysiological responses to three tested odorants. Furthermore, qPCR data revealed the inhibition of several olfactory receptor genes, including Orco. Immunohistochemistry showed that BdsNPFR was localized in cells under the sensillum on the antennae. Based on their shape and size, the BdsNPFR+ cells differ from odorant receptor neurons (ORNs), which were labeled using a Drosophila melanogaster Orco antibody. Our data suggest that sNPFR regulates olfaction-mediated foraging behavior by mediating interactions between BdsNPFR+ cells and selected ORNs.