Induction of pluripotent stem cells transplantation therapy for ischemic stroke
Induction of pluripotent stem cells transplantation therapy for ischemic stroke
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DOI：
10.1007/s11010-011-0806-5
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发表时间：
2011-08-01
影响因子：
4.3
通讯作者：
Dong, Qiang
中科院分区：
文献类型：
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作者：
Jiang, Mei;Lv, Lei;Dong, Qiang
中风可导致永久性神经损伤、并发症，甚至死亡。然而，没有任何治疗方法可以恢复其失去的功能。人胚胎干细胞移植治疗脑卒中是一种新的、有潜力的治疗方法。然而，正如我们所看到的，伦理争议涉及胚胎干细胞研究。人类诱导多能干细胞（iPSC）是最新一代的干细胞，可能是使用胚胎细胞争议的解决方案。在我们的研究中，我们通过引入四种定义的转录因子（Oct 4，Sox 2，Nanog和Lin-28）从成人成纤维细胞中产生iPSC。在此基础上，我们在大脑中动脉闭塞动物模型上研究了iPSCs移植治疗脑卒中的疗效。令人惊讶的是，我们发现移植的iPSCs迁移到受损的脑区，并成功分化为神经元样细胞。iPSCs移植4-16 d后，大鼠感觉运动功能明显改善。总之，我们可以证明iPSCs治疗中风是一种有效的治疗形式。
Stroke can cause permanent neurological damage, complications, and even death. However, there is no treatment exists to restore its lost function. Human embryonic stems transplantation therapy was a novel and potential therapeutic approach for stroke. However, as we have seen, the ethical controversy pertains to embryonic stem cell research. Human induced pluripotent stem cells (iPSCs) are the latest generation of stem cells that may be a solution to the controversy of using embryonic cells. In our study, we generated iPSCs from adult human fibroblasts by introduction of four defined transcription factors (Oct4, Sox2, Nanog, and Lin-28). And then, we investigated the efficacy of iPSCs transplantation therapy for stroke on the animal models of middle cerebral artery occlusion. Surprisingly, we found that transplanted iPSCs migrated to injured brain areas, and differentiated into neuron-like cells successfully. After 4-16 days iPSCs grafting, sensorimotor function of rats has been improved significantly. In one word, we may prove that iPSCs therapy in stroke to be an effective form of treatment.