UNBALANCED GROWTH IN A SEMI-CONTINUOUS CULTURE SYSTEM DESIGNED FOR SYNCHRONIZATION OF CELL DIVISION
UNBALANCED GROWTH IN A SEMI-CONTINUOUS CULTURE SYSTEM DESIGNED FOR SYNCHRONIZATION OF CELL DIVISION
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DOI：
10.1099/00221287-65-1-23
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发表时间：
1971-01-01
期刊：
影响因子：
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通讯作者：
ANAGNOSTOPOULOS, GD
中科院分区：
文献类型：
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作者：
ANAGNOSTOPOULOS, GD
在半连续培养系统中诱导大肠杆菌同步分裂。描述了为系统自动运行而设计的小型装置。每隔 1 至 4 小时供应碳（葡萄糖）限制确定培养基。排出一半稀释培养物。经过多次循环后，每次提供的葡萄糖刚好足以使“自建”群体翻倍。在新鲜培养基存在的情况下，每个周期开始时都会发生对数的活力损失，并且人们认为部分群体经历了不平衡的生长。这种现象和观察到存活种群由同步但在不同时间分裂的两个相等部分组成，被认为反映了分裂后新出现的两半种群在应对由进入的新鲜培养基引起的活跃生长方面的能力不平等。
Synchronization of division ofEscherichia coliwas induced in a semi-continuous culture system. A small-scale apparatus designed for the automatic operation of the system is described. The carbon (glucose)-limited defined medium was supplied at intervals of 1 to 4 h. and half of the diluted culture was discharged. After a number of cycles the glucose supplied each time was just sufficient for the doubling of the ‘self-established’ population. A logarithmic loss of viability occurred at the beginning of each cycle in the presence of fresh medium, and it was thought that part of the population underwent unbalanced growth. This phenomenon and the observation that the viable population consisted of two equal parts dividing synchronously but at different times were thought to reflect a post-division unequal competence of the emerging two halves of the population to cope with the active growth induced by the incoming fresh medium.