Plasma amino acid profiles at various reproductive stages in female rats
Plasma amino acid profiles at various reproductive stages in female rats
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DOI：
10.1292/jvms.15-0095
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发表时间：
2015-07-01
影响因子：
1.2
通讯作者：
Murakami, Noboru
中科院分区：
文献类型：
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作者：
Okame, Rieko;Nakahara, Keiko;Murakami, Noboru
我们测量了雌性大鼠各个生殖阶段（包括发情周期、怀孕和哺乳期）的血浆氨基酸水平，并使用二维或三维图形比较了所得的氨基酸谱。这些数据表明，怀孕和哺乳期母鼠的氨基酸谱与发情周期或雄性大鼠的氨基酸谱有很大不同。发情前期、发情期、发情后期和发情间期各氨基酸的血浆水平几乎相同，并且其分布没有显着差异。然而，母鼠怀孕和哺乳期间氨基酸谱发生了变化。妊娠中后期血浆Ser水平显着降低，Tyr、Gly、His在妊娠后期和末期显着降低，Trp、Lys在妊娠末期分别显着降低和升高。在哺乳期间观察到氨基酸谱发生了更大的变化，此时许多氨基酸的水平显着增加，并且没有一个显示出显着下降。血浆 Pro、Ser 和 Gly 水平从哺乳期第 1 天到第 15 天持续增加，而 Asn 和 Met 分别从第 1 天和第 5 天显着增加，直到哺乳期结束。这些结果表明，血浆氨基酸谱显示出根据生殖阶段的特征性变化，并且在进行基于氨基酸的诊断时可能有必要考虑这种差异。
We measured the plasma levels of amino acids at various reproductive stages in female rats, including the estrous cycle, pregnancy and lactation, and compared the resulting amino acid profiles using two- or three-dimensional figures. These figures revealed that the amino acid profiles of pregnant and lactating dams differed considerably from those during the estrous cycle or in male rats. The plasma levels of individual amino acids were almost the same between proestrus, estrus, metestrus and diestrus, and their profiles did not differ significantly. However, the amino acid profiles changed during pregnancy and lactation in dams. The plasma Ser level decreased significantly in mid and late pregnancy, whereas Tyr, Gly and His decreased significantly in the late and end stages of pregnancy, and Trp and Lys significantly decreased and increased at the end of pregnancy, respectively. Much larger changes in amino acid profiles were observed during lactation, when the levels of many amino acids increased significantly, and none showed a significant decrease. Plasma Pro, Ser and Gly levels increased continuously from day 1 until day 15 of lactation, whereas Asn and Met increased significantly from days 1 and 5 respectively until the end of lactation. These results suggest that the profiles of plasma amino acids show characteristic changes according to reproductive stage and that it may be necessary to consider such differences when performing amino acid-based diagnosis.