Determining simple sulfhydryl compounds (low molecular weight) and their contents in biological samples by using 2,2'-dithiobis-(5-nitropyridine).
Determining simple sulfhydryl compounds (low molecular weight) and their contents in biological samples by using 2,2'-dithiobis-(5-nitropyridine).
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使用 2,2-二硫双-(5-硝基吡啶) 测定生物样品中的简单巯基化合物（低分子量）及其含量。
DOI：
10.1016/0003-2697(72)90198-4
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发表时间：
1972
影响因子：
2.9
通讯作者：
C. C. Tsen
中科院分区：
文献类型：
--
作者：
A. Swatditat;C. C. Tsen
DTNP-2，2 ′-二硫代双-（5-硝基吡啶）-可用于生物样品中简单（低分子量）-SH化合物及其含量的分光光度测定。与5，5 ′-二硫代双（2-硝基苯甲酸）（DTNB）不同，DTNP在酸性或碱性介质（pH5.5 -8.9）中能与巯基化合物显色。显色稳定，因此适用于分析。DTNP比DTNB（一种广泛使用的显色剂）更灵敏，用于分光光度法测定-SH化合物。线性响应，符合比耳定律，吸光度读数的浓度，观察到所有的简单-SH化合物在研究中测试。在摩尔基础上，一旦这些-SH化合物与DTNP反应，它们给出基本相同的响应（在实验误差内）。用分光光度法和安培法测定生物样品中的简单巯基含量，两者之间有很高的相关性。
DTNP—2,2′-dithiobis-(5-nitropyridine)—was found to be useful for spectrophotometric determination of simple (low MW) -SH compounds and their contents in biological samples. Unlike 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), DTNP can develop color with -SH compounds at the acidic or alkaline pH medium (pH 5.5–8.9). The developed color is stable and, therefore, suitable for analysis. DTNP was found to be more sensitive than DTNB (a widely used color reagent) for spectrophotometric determination of -SH compounds. A linear response, conforming to Beer's law, of absorbance readings to concentrations was observed for all the simple -SH compounds tested in the study. On a molar basis, once those -SH compounds reacted with DTNP, they gave essentially identical responses (within experimental errors). A high correlation was demonstrated between the spectrophotometric and amperometric methods for determining simple -SH contents in biological samples.