Promoter variants at AP2 box region of Hsp70.1 affect thermal stress response and milk production traits in Frieswal cross bred cattle
Promoter variants at AP2 box region of Hsp70.1 affect thermal stress response and milk production traits in Frieswal cross bred cattle
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DOI：
10.1016/j.gene.2013.09.037
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发表时间：
2013-12-15
期刊：
影响因子：
3.5
通讯作者：
Sharma, Arjava
中科院分区：
文献类型：
--
作者：
Deb, Rajib;Sajjanar, Basavaraj;Sharma, Arjava
热休克蛋白（Heat shock proteins，Hsp）在保护细胞免受热应激中起重要作用。Hsp基因启动子区的核苷酸多态性影响Hsp mRNA的表达和诱导。本研究旨在探讨奶牛热休克蛋白70.1基因启动子区多态性对细胞表达量的影响，以及这些多态性与奶牛夏季应激生理指标和产奶性状的关系。采用双重PCR-RFLP对200头Frieswal奶牛进行基因分型，以确定Hsp70.1启动子AP 2框内碱基位置895处的胞嘧啶缺失。纯合野生型（CC）基因型（39.29，n = 78）低于杂合胞嘧啶缺失突变型（C）基因型（60.71，n = 122）。在所观察的生理参数（直肠温度、呼吸速率和耐热系数）中，纯合野生型奶牛的耐夏性显著（P < 0.05）优于杂合缺失型奶牛。启动子区胞嘧啶缺失突变对体外热应激牛外周血单个核细胞（PBMC）Hsp70.1mRNA的表达有负性影响（P < 0.01）。观察到的多态性与产奶性状的进一步关联是显著的，因为杂合胞嘧啶缺失奶牛的总产奶量、峰值产奶量、300日龄产奶量、蛋白质%（P < 0.01）和脂肪%（P < 0.05）低于天然野生型启动子奶牛。本研究结果表明，牛hsp70.1基因启动子区具有多态性，可用于奶牛耐热性和产奶量的选择。(C)2013爱思唯尔有限公司版权所有。
Heat shock proteins (Hsp) are known to play major role in protection of cells from thermal stress. Nucleotide polymorphisms within the promoter of Hsp affect degree of expression and inducibility of Hsp mRNA. The present study aimed to investigate the effect of polymorphism within promoter region on the cellular expression of Hsp70.1 mRNA and association of identified polymorphisms with the physiological parameters during summer stress and milk production traits in dairy cattle. Two hundred Frieswal cows were genotyped using double PCR-RFLP to identify deletion of cytosine within the Hsp70.1 promoter AP2 box at base position 895. Homozygous wild type genotypes (CC) were found in lower frequency (39.29, n = 78) than heterozygous cytosine deletion mutant genotypes (C) (60.71, n = 122). In the observed physiological parameters (rectal temperature, respiration rate and heat tolerance coefficient), cows that were homozygous wild types had better significant (P < 0.05) summer tolerance than the heterozygous deletion genotypes. Cytosine deletion mutation in the promoter region negatively affected (P < 0.01) the expression of Hsp70.1 mRNA in peripheral bovine mononuclear cells (PBMC) subjected to in vitro heat stress. Further association of observed polymorphism with the milk production traits was significant as the heterozygous cytosine deletion cows had lower total milk yield, peak yield, yield at 300 days, protein% (P < 0.01) and fat% (P < 0.05) than the native wild type promoter cows. The results from the present study suggest that the promoter region of bovine hsp70.1 gene is polymorphic and may be useful in selection of dairy cows for relatively better thermotolerance and higher milk production. (C) 2013 Elsevier B.V. All rights reserved.