Structural analysis of reactionary dentin formed in response to polymicrobial invasion.
Structural analysis of reactionary dentin formed in response to polymicrobial invasion.
复制标题
DOI：
10.1016/j.jsb.2012.12.005
复制
发表时间：
2013-03
影响因子：
3
通讯作者：
Hunter, Neil
中科院分区：
文献类型：
--
作者：
Charadram, Nattida;Austin, Christine;Trimby, Patrick;Simonian, Mary;Swain, Michael V.;Hunter, Neil
当微生物侵入牙本质时，成牙本质细胞分泌一种改变的钙化基质，称为反应性牙本质（Rd）。聚焦离子束扫描电子显微镜（FIB-SEM）图像切片的3D重建显示Rd中的螺旋管状结构，与健康牙齿的牙本质特征的规则圆柱形小管形成对比，并影响位于Rd外部的所谓生理性牙本质（Pd）。Rd中的这种螺旋结构提供了对小管腔直径的有效收缩，从而形成了细菌向牙髓前进的屏障。树脂铸型的扫描电镜显示，通过Rd的成牙本质细胞的过程改变的延伸。结合聚焦离子束X射线微区分析（FIB EDS）、激光烧蚀电感耦合等离子体质谱（LA-ICP-MS）和漫反射红外傅里叶变换光谱（DRIFTS）技术，对关键矿物元素的分布进行了研究。有一个显着的重新分配的钙和磷在Rd一起增加的扩散沉积镁兼容的矿物沉积相合成的这种改变矩阵。小管结构和矿物质含量特征的Rd的变化是一致的，降低硬度和较低的弹性模量，该矩阵的报告。研究结果提供了深入了解的独特结构的Rd合成作为一个主要的感染反应。
In response to microbial invasion of dentin odontoblasts secrete an altered calcified matrix termed reactionary dentin (Rd). 3D reconstruction of focused-ion-beam scanning electron microscopy (FIB-SEM) image slices revealed helical tubular structures in Rd that contrasted with regular cylindrical tubules characteristic of dentin from healthy teeth and affected so-called physiological dentin (Pd) lying exterior to Rd. This helical structure in Rd provided effective constriction of tubule lumen diameter that formed a barrier to bacterial advance towards the dental pulp. SEM of resin cast preparations revealed altered extension of odontoblast processes through Rd. The distribution of key mineral elements was studied by combination of 3D reconstruction of focused-ion-beam based X-ray microanalysis (FIB-EDS), laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) and diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS). There was a marked redistribution of calcium and phosphorous in Rd together with an increase of diffusely deposited magnesium compatible with the mineral deposition phase of synthesis of this altered matrix. Changes in tubule structure and mineral content characteristic of Rd are consistent with reduced hardness and lower elastic modulus reported for this matrix. Findings provide insight into the unique structure of Rd synthesised as a primary response to infection.
登录
查看更多内容
DOI：
10.1002/tera.1420440410
发表时间：
1991-10-01
期刊：
TERATOLOGY
影响因子：
--
作者：
FERREIRA, RMCD;GONZALEZ, JLR;ELIZAGA, IV
通讯作者：
ELIZAGA, IV
影响因子：
4.8
作者：
Greene, EF;Tauch, S;Amarasiriwardena, D
通讯作者：
Amarasiriwardena, D
影响因子：
3.3
作者：
Dziak, Katherine L.;Akkus, Ozan
通讯作者：
Akkus, Ozan
影响因子：
6
作者：
Farahani, Ramin M.;Nguyen, Ky-Anh;Hunter, Neil
通讯作者：
Hunter, Neil
影响因子：
3
作者：
Kinney, JH;Balooch, M;Marshall, SJ
通讯作者：
Marshall, SJ