Influence of monostrain and multistrain probiotics on immunity, intestinal ultrastructure and microbiota in experimental dysbiosis
Influence of monostrain and multistrain probiotics on immunity, intestinal ultrastructure and microbiota in experimental dysbiosis
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DOI：
10.3920/bm2017.0117
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发表时间：
2018-01-01
影响因子：
5.4
通讯作者：
Suvorov, A.
中科院分区：
文献类型：
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作者：
Ermolenko, E.;Rybalchenko, O.;Suvorov, A.
利用抗生素诱导的大鼠生态失调模型，研究了三种益生菌菌株鼠李糖乳杆菌 K32、长双歧杆菌 GT15、屎肠球菌 L3 及其混合物的生物学效应。研究发现，服用不同的益生菌后，肠道微生物群会以菌株特异性的方式发生变化。在施用所研究的细菌菌株混合物或单一肠球菌菌株后，显示了针对病原体的最大活性。屎肠球菌 L3 菌株对两种克雷伯氏菌均表现出最强的活性。和脆弱拟杆菌。它有助于恢复普氏粪杆菌的原始含量。克雷伯氏菌属的数量。接受鼠李糖乳杆菌 K32 的组和不食用益生菌的动物组的情况相同。组合物中包含的不同益生菌菌株具有不同的免疫学作用。益生菌双歧杆菌、肠球菌和三种益生菌的混合物刺激肠系膜淋巴结中白细胞介素 (IL)-10 的 mRNA 表达。食用肠球菌益生菌后微生物群的变化与血清中转化生长因子 (TGF)-β 和 IL-10 含量的增加以及肠粘液层的增加相关。食用鼠李糖乳杆菌 K32 会刺激肠系膜淋巴结中 IL-8 的表达。未接受益生菌的对照组的特征是促炎细胞因子的表达、上皮细胞的损伤及其紧密连接的破坏。所有服用益生菌的组都预防了粘膜超微结构的损伤。
The biological effects of three probiotic strains Lactobacillus rhamnosus K32, Bifidobacterium longum GT15, Enterococcus faecium L3 and their mixture were studied using a model of dysbiosis induced in rats by antibiotics. It was found that after taking different probiotics intestinal microbiota changed in a strain-specific manner. The maximal activity against pathogens was revealed after the administration of a mixture of bacterial strains under study or a single strain of enterococci. The strain E. faecium L3 showed the most activity against both Klebsiella spp. and Bacteroides fragilis. It helped to restore the original content of Faecalibacterium prausnitzii. The number of Klebsiella spp. was the same in the group receiving L. rhamnosus K32 and the group of animals, which was not consuming probiotics. Different probiotic strains included in the composition had various immunological effects. Probiotic bifidobacteria, enterococci and the mixture of three probiotics stimulated of mRNA expression of interleukin (IL)-10 in mesenteric lymph nodes. The changes in microbiota after consuming an enterococcal probiotic correlated with an increase in transforming growth factor (TGF)-beta and IL-10 content in blood serum and an increase of the intestinal mucus layer. Consumption of L. rhamnosus K32 led to the stimulation of IL-8 expression in mesenteric lymph nodes. Control group not receiving probiotics was characterised by expression of pro-inflammatory cytokines, damage of epithelial cells and the destruction of their tight junctions. The damage to the ultrastructure of the mucosa was prevented in all the groups taking probiotics.