A low-cost multielectrode system for data acquisition enabling real-time closed-loop processing with rapid recovery from stimulation artifacts.
A low-cost multielectrode system for data acquisition enabling real-time closed-loop processing with rapid recovery from stimulation artifacts.
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DOI：
10.3389/neuro.16.012.2009
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Potter SM
中科院分区：
文献类型：
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作者：
Rolston JD;Gross RE;Potter SM
用于自由活动动物多电极记录的市售数据采集系统价格昂贵，通常依赖于专有软件，并且不提供详细的、可修改的电路原理图。当与电刺激结合使用时，它们容易产生长时间的、饱和的刺激伪影，从而妨碍记录短延迟诱发反应。然而，电刺激是许多实验设计不可或缺的一部分，对于新兴的脑机接口和神经假体应用至关重要。为了解决这些问题，我们开发了一种易于使用、可修改且廉价的多电极神经记录和刺激系统。 64 个通道的设置成本低于 10,000 美元，比同类商业系统低一个数量级。与商业设备不同，该系统可以从刺激中快速恢复，并允许检测短延迟动作电位（刺激后<1毫秒），从而促进闭环应用并暴露原本隐藏的神经活动。为了说明这种能力，提出了啮齿动物海马体的微刺激诱发的活动。系统噪声水平与现有平台相似，并且可以同时记录细胞外动作电位和局部场电位。该系统采用模块化设计，有 16 个通道，使用灵活：虽然主要设计用于体内使用，但它可以与商用前置放大器结合使用，以从体外多电极阵列进行记录。该系统的开源控制软件NeuroRighter是用C#实现的，具有易于使用的图形界面。由于 C# 在托管代码环境中运行，这可能会影响性能，因此进行了分析以确保该应用程序的速度与 C++ 相当。硬件原理图、布局文件和软件均可免费获得。由于与自由移动的动物保持有线探头连接很困难，我们描述了一种使用钕磁铁进行电极-探头耦合的新方法。
Commercially available data acquisition systems for multielectrode recording from freely moving animals are expensive, often rely on proprietary software, and do not provide detailed, modifiable circuit schematics. When used in conjunction with electrical stimulation, they are prone to prolonged, saturating stimulation artifacts that prevent the recording of short-latency evoked responses. Yet electrical stimulation is integral to many experimental designs, and critical for emerging brain-computer interfacing and neuroprosthetic applications. To address these issues, we developed an easy-to-use, modifiable, and inexpensive system for multielectrode neural recording and stimulation. Setup costs are less than US$10,000 for 64 channels, an order of magnitude lower than comparable commercial systems. Unlike commercial equipment, the system recovers rapidly from stimulation and allows short-latency action potentials (<1 ms post-stimulus) to be detected, facilitating closed-loop applications and exposing neural activity that would otherwise remain hidden. To illustrate this capability, evoked activity from microstimulation of the rodent hippocampus is presented. System noise levels are similar to existing platforms, and extracellular action potentials and local field potentials can be recorded simultaneously. The system is modular, in banks of 16 channels, and flexible in usage: while primarily designed for in vivo use, it can be combined with commercial preamplifiers to record from in vitro multielectrode arrays. The system's open-source control software, NeuroRighter, is implemented in C#, with an easy-to-use graphical interface. As C# functions in a managed code environment, which may impact performance, analysis was conducted to ensure comparable speed to C++ for this application. Hardware schematics, layout files, and software are freely available. Since maintaining wired headstage connections with freely moving animals is difficult, we describe a new method of electrode-headstage coupling using neodymium magnets.