In vivo degradation of porous poly(propylene fumarate)/poly(DL-lactic-co-glycolic acid) composite scaffolds
In vivo degradation of porous poly(propylene fumarate)/poly(DL-lactic-co-glycolic acid) composite scaffolds
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DOI：
10.1016/j.biomaterials.2004.11.039
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发表时间：
2005-08-01
期刊：
影响因子：
14
通讯作者：
Jansen, JA
中科院分区：
文献类型：
--
作者：
Hedberg, EL;Kroese-Deutman, HC;Jansen, JA
研究了聚富马酸丙二醇酯（PPF）/聚（DL-乳酸-羟基乙酸）共聚物（PLGA）成骨因子缓释复合支架的体内降解。将PPF/PLGA复合材料植入兔桡骨15.0mm节段性缺损中，12周和18周后收获，并使用组织学技术进行分析，以评估聚合物降解的程度以及支架孔内的组织反应。在12周和18周时，聚合物降解仅限于植入物末端和边缘支架的微碎片。支架孔隙内的组织由纤维组织、血管和一些炎性细胞组成。在聚合物分解明显的区域，观察到炎症反应增加。相反，骨长入聚合物支架的区域的特征在于最小的炎症反应和聚合物降解。我们的研究结果表明，多孔PPF在兔模型中植入18周内发生最小降解。此外，多孔PPF/PLGA支架的体内降解数据与早期获得的体外数据相当。(c)2004 Elsevier Ltd.保留所有权利。
This study investigated the in vivo degradation of poly(propylene fumarate) (PPF)/poly(DL-lactic-co-glycolic acid) (PLGA) composite scaffolds designed for controlled release of osteogenic factors. PPF/PLGA composites were implanted into 15.0mm segmental defects in the rabbit radius, harvested after 12 and 18 weeks, and analyzed using histological techniques to assess the extent of polymer degradation as well as the tissue response within the pores of the scaffolds. Polymer degradation was limited to micro-fragmentation of the scaffold at the ends and edges of the implant at both 12 and 18 weeks. The tissue within the pores of the scaffold consisted of fibrous tissue, blood vessels and some inflammatory cells. In areas where polymer breakdown was evident, an increased inflammatory response was observed. In contrast, areas of bone ingrowth into the polymer scaffold were characterized by minimal inflammatory response and polymer degradation. Our results show that minimal degradation of porous PPF occurs within 18 weeks of implantation in a rabbit model. Further, the in vivo degradation data of porous PPF/PLGA scaffolds are comparable with earlier obtained in vitro data. (c) 2004 Elsevier Ltd. All rights reserved.