Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion
Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion
复制标题
DOI：
10.1038/nm.2294
复制
发表时间：
2011-02-01
期刊：
影响因子：
82.9
通讯作者：
Nishida, Noriyuki
中科院分区：
文献类型：
--
作者：
Atarashi, Ryuichiro;Satoh, Katsuya;Nishida, Noriyuki
体外扩增异常构象朊病毒蛋白（PrPSc）技术的发展产生了潜在的敏感检测朊病毒。在这里，我们开发了一种新的PrPSc扩增测定，称为实时振荡诱导转换（RT-QUIC），它允许检测稀释的克雅氏病（CJD）脑匀浆中>= 1 fg的PrPSc。此外，我们首先在一系列日本受试者中评估了该技术，然后在来自澳大利亚的30份脑脊液标本的盲法研究中评估了该技术，其灵敏度超过80%，特异性超过100%。这些研究结果表明，有前途的增强诊断能力的RT-QUIC在疑似CJD的死前评估。
The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrPSc) has generated the potential for sensitive detection of prions. Here we developed a new PrPSc amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of >= 1 fg of PrPSc in diluted Creutzfeldt-Jakob disease (CJD) brain homogenate. Moreover, we assessed the technique first in a series of Japanese subjects and then in a blind study of 30 cerebrospinal fluid specimens from Australia, which achieved greater than 80% sensitivity and 100% specificity. These findings indicate the promising enhanced diagnostic capacity of RT-QUIC in the antemortem evaluation of suspected CJD.