Impairment of thrombospondin-1 expression during epithelial wound healing in corneas of vitamin A-deficient mice.
Impairment of thrombospondin-1 expression during epithelial wound healing in corneas of vitamin A-deficient mice.
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DOI：
10.14670/hh-20.493
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发表时间：
2005-04
影响因子：
2
通讯作者：
Koichi Uno;Masahide Kuroki;Hideyuki Hayashi;H. Uchida;Kenji Oshima
中科院分区：
文献类型：
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作者：
Koichi Uno;Masahide Kuroki;Hideyuki Hayashi;H. Uchida;Kenji Oshima
本研究的目的是研究血小板反应蛋白-1（TSP-1），一种多功能的细胞外基质蛋白，在维生素A缺乏小鼠角膜损伤的再上皮化过程中的表达。用微研磨机在正常和维生素A缺乏小鼠的角膜中产生上皮缺损。用荧光素对受伤的角膜进行染色并拍照以评价上皮再生。组织学检查和免疫组化分析TSP-1的表达，从受伤的角膜标本进行。在维生素A缺乏的小鼠中，与正常小鼠相比，受伤的角膜上皮的再上皮化显著延迟。TSP-1在正常小鼠未受伤的角膜上皮和维生素A缺乏小鼠的角膜上皮中均未检测到。在正常小鼠中，分别在磨损后30 min和8 h至16 h在受伤的角膜表面和基质上观察到TSP-1的线性染色，并且在36 h至48 h，当再上皮化完成时，TSP-1的表达消失。相比之下，在整个伤口愈合过程中，在维生素A缺乏小鼠的受伤角膜中没有观察到TSP-1染色，除了内皮细胞。组织学检查显示，在愈合过程中，维生素A缺乏小鼠角膜基质中的多形核中性粒细胞浸润逐渐增加。这些发现表明，维生素A可以调节角膜中TSP-1的表达，以加速受伤角膜的上皮再生。
The purpose of this study is to investigate the expression of thrombospondin-1 (TSP-1), a multifunctional extracellular matrix protein, during re-epithelialization in wounded corneas of vitamin A-deficient mice. Epithelial defects were created in the corneas of normal and Vitamin A-deficient mice with a microgrinder. Wounded corneas were stained with fluorescein and photographed for evaluation of re-epithelialization. Histological examination and immunohistochemical analysis of TSP-1 expression were also performed on the specimens from wounded corneas. In vitamin A-deficient mice, re-epithelialization of the wounded corneal epithelium was significantly delayed compared with that in normal mice. TSP-1 was detectable neither in the unwounded corneal epithelium of normal mice nor in that of vitamin A-deficient mice. In normal mice, linear staining of TSP-1 was observed on the wounded corneal surface and stroma at 30 min and 8 h to 16 h, respectively, after abrasion, and this TSP-1 expression disappeared at 36 to 48 h, when re-epithelialization was completed. In contrast, no TSP-1 staining was observed in the wounded corneas of vitamin A-deficient mice, except for the endothelial cells, throughout the wound healing process. Histological examination revealed a progressive increase in polymorphonuclear neutrophil infiltration in the stroma of the corneas of vitamin A-deficient mice during the healing process. These findings suggest that vitamin A may modulate the expression of TSP-1 in the corneas to accelerate the re-epithelialization of wounded corneas.