Spatial organization of the bipolar cell's receptive field in the retina of the tiger salamander.
Spatial organization of the bipolar cell's receptive field in the retina of the tiger salamander.
复制标题
虎蝾螈视网膜中双极细胞感受野的空间组织。
DOI：
10.1113/jphysiol.1990.sp017942
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Owen,WG
中科院分区：
文献类型：
--
作者：
Hare,WA;Owen,WG
1.在离体灌流的虎蝾螈视网膜上，用细胞内记录技术研究了视杆细胞、水平细胞和双极细胞的空间特性。使用低刺激强度以保持细胞响应接近或在其线性强度/响应范围内。2.双极细胞感受野的空间特性，测量，而灌注正常林格氏溶液，进行了比较，与那些在暴露于消除双极细胞的感受野周围（RFS）的代理人。通过这种方式，可以独立地表征感受野中心（RFC）和RFS的空间特性。3.水平细胞感受野单位面积对反应的贡献随离感受野中心的距离呈指数下降。描述这种衰减的（表观）长度常数为200微米。因此，水平细胞合胞体的一维长度常数为248微米。反应幅度随中心圆形刺激半径的变化与这一发现一致。4.双极细胞的RFC也是如此。偏心双极电池RFC的一维长度常数平均为124微米。中心单元的RFC平均值为62微米，尽管数值变化更大，一些中心单元的RFC与偏离中心单元的RFC相当。这些值是独立的类感光驱动双极细胞。5.偏离中心细胞和许多中心细胞的RFC的大尺寸不能通过视网膜内的光散射或杆合胞体内的电压分布来解释。我们提出偏心细胞在合胞体中紧密耦合。平均而言，中心细胞的耦合较不紧密。6.双极细胞的RFS的空间特性与RFS代表水平细胞的感受野和双极细胞的RFC的卷积的概念一致。7.双极细胞感受野的空间特性是由其RFC的测量特性和水平细胞感受野的测量特性重建的。在我们的实验条件下，双极细胞的响应可以描述由RFC产生的组件和RFS产生的组件之间的线性差。8.根据我们的数据计算了双极细胞的空间滤波特性。（400字处截断摘要）
1. The spatial properties of rods, horizontal cells and bipolar cells were studied by intracellular recording in the isolated, perfused retina of the tiger salamander, Ambystoma tigrinum. Low stimulus intensities were used in order to keep cell responses close to, or within, their linear intensity/response range. 2. Spatial properties of bipolar cell receptive fields, measured while perfusing with normal Ringer solution, were compared with those measured during exposure to agents that eliminated the bipolar cells' receptive field surround (RFS). In this way, the spatial properties of the receptive field centre (RFC) and those of the RFS could be characterized independently. 3. To a good approximation, the contribution to the horizontal cell's response of unit area of its receptive field declined exponentially with distance from the centre of the receptive field. The (apparent) length constant describing this decay was 200 microns. The one‐dimensional length constant of the horizontal cell syncytium was thus 248 microns. The variation of response amplitude with the radius of a centred circular stimulus was consistent with this finding. 4. This was true also of the RFCs of bipolar cells. The one‐dimensional length constant of the RFC of off‐centre bipolar cells averaged 124 microns. That of the RFC of on‐centre cells averaged 62 microns though values were more variable, the RFCs of some on‐centre cells being comparable to those of off‐centre cells. These values were independent of the class of photoreceptor driving the bipolar cell. 5. The large size of the RFCs of off‐centre cells and many on‐centre cells cannot by explained by light scatter within the retina or by voltage spread within the rod syncytium. We proposed that off‐centre cells are tightly coupled in a syncytium. On‐centre cells, on average, are less tightly coupled. 6. The spatial properties of the bipolar cell's RFS were consistent with the notion that the RFS represents a convolution of the horizontal cell's receptive field and the bipolar cell's RFC. 7. The spatial properties of bipolar cell receptive fields were reconstructed from the measured properties of their RFCs and the measured properties of horizontal cell receptive fields. Under the conditions of our experiments, the bipolar cell's response could be described by a linear difference between a component generated by the RFC and a component generated by the RFS. 8. The spatial filtering characteristics of the bipolar cells were calculated from our data.(ABSTRACT TRUNCATED AT 400 WORDS)