In vivo laser confocal microscopy findings of cryopreserved and fresh human amniotic membrane.
In vivo laser confocal microscopy findings of cryopreserved and fresh human amniotic membrane.
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DOI：
10.3928/15428877-20080701-10
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发表时间：
2008-07
期刊：
影响因子：
--
通讯作者：
A. Kobayashi;K. Sugiyama;Wei Li;S. Tseng
中科院分区：
文献类型：
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作者：
A. Kobayashi;K. Sugiyama;Wei Li;S. Tseng
背景与目的近年来，角膜特异的活体激光共聚焦显微镜已经问世。本研究的目的是获得新鲜和冷冻保存的人羊膜的激光共聚焦图像。材料与方法用活体激光扫描共聚焦显微镜(Heidelberg Retina Tomograph II Rostock Cornea模块[HRT II-RCM]；Heidelberg Engineering GmbH，Dossenheim，Dossenheim)对新鲜羊膜和冷冻保存的不同供体羊膜进行观察。结果在新鲜和冷冻羊膜标本中，HRT II-RCM可分辨出4个不同的层：上皮、基底膜、富含细胞的间质层(成纤维细胞层)和海绵状间质层。在基底膜的后部(面向成纤维细胞层)，新鲜羊膜的微皱折比冷冻保存的羊膜更明显。对于富含细胞的间质层，新鲜羊膜中的间质细胞比冷冻保存的羊膜中的间充质细胞更明显。结论这些基线激光共聚焦图像可用于进一步研究羊膜移植后的体内重塑。
BACKGROUND AND OBJECTIVE Recently, the cornea-specific in vivo laser confocal microscope has become available. The objective of the current study was to obtain laser confocal images of fresh and cryopreserved human amniotic membrane. MATERIALS AND METHODS Fresh and cryopreserved amniotic membranes from different donors were examined using the in vivo laser scanning confocal microscope (Heidelberg Retina Tomograph II Rostock Cornea Module [HRT II-RCM]; Heidelberg Engineering GmbH, Dossenheim, Germany). RESULTS In all samples of both fresh and cryopreserved amniotic membranes, the HRT II-RCM resolved four distinct layers: the epithelium, a basement membrane, a cell-rich stromal layer (fibroblast layer), and a spongy stromal layer. In a posterior aspect of the basement membrane layer (facing the fibroblast layer), microfolds were more prominent in fresh amniotic membranes than in cryopreserved amniotic membranes. For the cell-rich stromal layer, mesenchymal cells were observed more distinctively in fresh amniotic membranes than in cryopreserved amniotic membranes. CONCLUSION These baseline laser confocal images can be used for future investigation of in vivo remodeling after amniotic membrane transplantation.