Structure, expression, and hormonal control of genes from the mosquito, Aedes aegypti, which encode proteins similar to the vitelline membrane proteins of Drosophila melanogaster.
Structure, expression, and hormonal control of genes from the mosquito, Aedes aegypti, which encode proteins similar to the vitelline membrane proteins of Drosophila melanogaster.
复制标题
埃及伊蚊基因的结构、表达和激素控制，这些基因编码与黑腹果蝇卵黄膜蛋白相似的蛋白质。
DOI：
10.1006/dbio.1993.1052
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发表时间：
1993
影响因子：
2.7
通讯作者：
Hagedorn,HH
中科院分区：
文献类型：
--
作者：
Lin,Y;Hamblin,MT;Edwards,MJ;Barillas-Mury,C;Kanost,MR;Knipple,DC;Wolfner,MF;Hagedorn,HH
Genomic and cDNA clones of a gene expressed after a blood meal in the mosquito,Aedes aegypti, were identified as having significant similarity to the vitelline membrane protein genes ofDrosophila melanogaster. The predicted protein had unusually high contents of alanine, histidine, and proline and contained a region of hydrophobic amino acids that was highly conserved in the predicted protein of theD. melanogastervitelline membrane protein genes. The 15a gene was expressed from 5 to 40 hr after a blood meal. It was expressed only in the follicle cells of the ovary, particularly in the cells surrounding the oocyte. The 15a gene was expressed in ovaries of the blood-fed, decapitated female in response to an injection of 20-hydroxyecdysone, and in ovaries from non-blood-fed females incubated with the hormone, even in the presence of cycloheximide. A second gene, with weaker homology to 15a, is presumably another member of a family of related genes, as is the case withD. melanogastervitelline membrane protein genes. This second gene contained a coding sequence similar to a decapeptide recently isolated from mosquito ovaries as an "oostatic factor" (Borovsky et el., FASEB J. 4, 3015-3020, 1990).
