Retrovirus infection of cells in vitro and in vivo.
Retrovirus infection of cells in vitro and in vivo.
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DOI：
10.1002/0471142727.mb0914s36
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发表时间：
2001-05-01
影响因子：
--
通讯作者：
Pear, W
中科院分区：
文献类型：
--
作者：
Cepko, C;Pear, W
逆转录病毒载体用作转导剂有许多应用。在某些情况下，载体携带希望在靶细胞中表达的基因，以便研究该基因的功能。在其他情况下，病毒被用来将组织化学标记基因引入细胞，以跟踪它们的命运。逆转录病毒载体也可用于多种细胞类型中以研究调控序列，其中载体携带报道基因和调控序列，并通过癌基因的转导使原代细胞永生化或转化原代细胞。对于每个应用程序，感染协议可能会有所不同，并且必须经常进行优化。在一些典型的体内和体外实验中的细胞感染的指导方针在此概述。
There are many applications in which retrovirus vectors are used as transduction agents. In some cases, the vector carries a gene that one wishes to express in a target cell in order to study the function of that gene. In other cases, the virus is used to introduce a histochemical marker gene into cells in order to follow their fate. Retrovirus vectors can also be used in a variety of cells type to investigate regulatory sequences in which a reporter gene and regulatory sequences are carried by the vector and to immortalize or transform primary cells by transduction of oncogenes. For each application, the infection protocol may vary and must often be optimized. Guidelines for infection of cells in some typical in vivo and in vitro experiments are presented in this overview.