Investigation of flavonoid profile of Scutellaria bacalensis Georgi by high performance liquid chromatography with diode array detection and electrospray ion trap mass spectrometry
Investigation of flavonoid profile of Scutellaria bacalensis Georgi by high performance liquid chromatography with diode array detection and electrospray ion trap mass spectrometry
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DOI：
10.1016/j.chroma.2009.04.021
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发表时间：
2009-06-05
影响因子：
4.1
通讯作者：
Yao, Shouzhuo
中科院分区：
文献类型：
--
作者：
Liu, Guozhu;Ma, Jinyu;Yao, Shouzhuo
黄芩是一种著名的药用植物，在中国和其他东亚国家广泛使用。采用高效液相色谱结合二极管阵列检测和电喷雾离子阱质谱测定黄芩黄酮成分。在优化的实验条件下，清晰地检出32种黄酮类化合物。通过将保留时间、UV 和 NIS (MSn) 数据与孤立的或商业标准进行比较，无疑可以确定 18 个主要的数据。对这 18 个已知标准的 UV 特性进行了脱轨研究。总结的规则为后续的在线识别过程提供了有价值的指导。通过详细解释 MS 和 UV 数据，成功在线鉴定了黄芩中的其他 13 种微量黄酮类化合物。 (C) 2009 Elsevier B.V. 保留所有权利。
Scutellaria baicalensis Georgi is a well-known medicinal plant widely used in China and other East Asian countries. High performance liquid chromatography combined with diode array detection and electrospray ion trap mass spectrometry Was used to determine the flavonoid profile of S. baicalensis. Under the optimized experiment conditions, 32 flavonoids were clearly detected. Eighteen main ones were doubtless identified by comparing their retention time, UV and NIS (MSn) data with isolated or commercial standards. The UV characteristics of these 18 known standards were studied in derail. The rules summarized provided valuable indications for the Subsequent on-line identification processes. By interpreting both the MS and the UV data in detail, other 13 minor flavonoids in S. baicalensis were on-line identified successfully. (C) 2009 Elsevier B.V. All rights reserved.