Production of ethanol from cellulosic biomass hydrolysates using genetically engineered Saccharomyces yeast capable of cofermenting glucose and xylose
Production of ethanol from cellulosic biomass hydrolysates using genetically engineered Saccharomyces yeast capable of cofermenting glucose and xylose
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DOI：
10.1385/abab:114:1-3:403
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发表时间：
2004-03-01
影响因子：
3
通讯作者：
Ho, NWY
中科院分区：
文献类型：
--
作者：
Sedlak, M;Ho, NWY
最近的研究证明乙醇是运输的理想液体燃料，而可再生木质纤维素材料是通过发酵生产乙醇燃料的有吸引力的原料。大多数纤维素生物质水解产生的主要可发酵糖是 D-葡萄糖和 D-木糖。工业上用淀粉和蔗糖生产乙醇的天然酵母菌不能代谢木糖。我们普渡大学的研究小组成功开发了基因工程酵母菌，能够有效地将葡萄糖和木糖共同发酵为乙醇，这是通过将三个木糖代谢基因克隆到酵母中来实现的。在这项研究中，我们证明了我们的稳定重组酵母424A（LNH-ST）含有以高拷贝数稳定整合到酵母染色体中的克隆木糖代谢基因，可以有效地将不同纤维素生物质水解产物中存在的葡萄糖和木糖发酵为乙醇。
Recent studies have proven ethanol to be the ideal liquid fuel for transportation, and renewable lignocellulosic materials to be the attractive feedstocks for ethanol fuel production by fermentation. The major fermentable sugars from hydrolysis of most cellulosic biomass are D-glucose and D-xylose. The naturally occurring Saccharomyces yeasts that are used by industry to produce ethanol from starches and cane sugar cannot metabolize xylose. Our group at Purdue University succeeded in developing genetically engineered Saccharomyces yeasts capable of effectively cofermenting glucose and xylose to ethanol, which was accomplished by cloning three xylose-metabolizing genes into the yeast. In this study, we demonstrated that our stable recombinant Saccharomyces yeast, 424A(LNH-ST), which contains the cloned xylose-metabolizing genes stably integrated into the yeast chromosome in high copy numbers, can efficiently ferment glucose and xylose present in hydrolysates from different cellulosic biomass to ethanol.