Activity-dependent glial swelling is impaired in aquaporin-4 knockout mice
Activity-dependent glial swelling is impaired in aquaporin-4 knockout mice
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DOI：
10.1016/j.neures.2009.03.002
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发表时间：
2009-06-01
影响因子：
2.9
通讯作者：
Nakada, Tsutomu
中科院分区：
文献类型：
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作者：
Kitaura, Hiroki;Tsujita, Mika;Nakada, Tsutomu
我们研究了水通道蛋白-4（AQP 4），一种在神经胶质细胞中表达的水通道，在脑切片制备中观察到的神经活动介导的形态学变化中的作用。黄素蛋白荧光（FF）和红外光散射（LS）信号的变化进行了测量之前和之后的重复刺激层VI在嘴侧体感皮层切片从AQP 4基因敲除（KO）和野生型（WT）小鼠。反映神经有氧活动的FF变化在所有皮质层中对两组具有可比性。然而，与WT小鼠相比，AQP 4 KO小鼠第1层中LS信号的变化（指示细胞肿胀）显著降低。我们的结论是，AQP 4可能在神经活性依赖性胶质细胞肿胀中发挥重要作用。(C)2009年爱思唯尔爱尔兰有限公司和日本神经科学学会。All rights reserved.
We investigated the role of aquaporin-4 (AQP4), a water channel expressed in glial cells, in neural activity mediated morphological changes observed in brain slice preparation. Changes in flavoprotein fluorescence (FF) and infrared light scattering (LS) signals were measured before and after repetitive stimulation of layer VI in rostral somatosensory cortical slices taken from AQP4 knockout(KO)and wildtype (WT) mice. Changes in FF, which reflect neural aerobic activities, were comparable for the two groups in all cortical layers. However, changes in LS signals, which are indicative of cell swelling, were significantly decreased in layer 1 of AQP4 KO mice compared to that of WT mice. We conclude that AQP4 likely plays a significant role in neural activity-dependent glial swelling. (C) 2009 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.