Ion channels activated by inositol 1,4,5-trisphosphate in plasma membrane of human T-lymphocytes
Ion channels activated by inositol 1,4,5-trisphosphate in plasma membrane of human T-lymphocytes
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人 T 淋巴细胞质膜中肌醇 1,4,5-三磷酸激活的离子通道
作者：
M. Kuno;P. Gardner
膜结合磷脂酰肌醇4，5-二磷酸(PtdIns(4，5)-P2)水解为水溶性的1，4，5-三磷酸(Ins(L，4，5)P3)是许多不同种类的细胞对各种外界刺激的共同反应(见文献1和2)。Ins(1，4，5)P3是一种可能的第二信使，它通过动员细胞内的钙库来增加细胞内的钙离子，这一假说已被对化学通透性细胞3-13和分离的微粒体膜组分11，12-16的研究证实。但Ins(1，4，5)P3可以通过跨膜通道在完整细胞中诱导外部钙离子内流的可能性从未被直接验证过，最初是由Micell在197517提出的假设。在此，我们报道了切除的T淋巴细胞膜上Ins(1，4，5)P3激活的电导的单通道记录。INS(1，4，5)P3激活的跨膜通道似乎与最近描述的参与T细胞激活的有丝分裂原调节的、电压不敏感的钙离子通透通道相同。我们认为INS(1，4，5)P3在丝裂原刺激的T细胞活化过程中起第二信使的作用，通过特定的钙离子通透通道调节跨膜钙内流。
Hydrolysis of membrane-associated phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)-P2) to water soluble inositol 1,4,5-trisphosphate (Ins(l,4,5)P3) is a common response by many different kinds of cells to a wide variety of external stimuli (see refs 1 and 2 for review). Ins (1,4,5)P3 is a putative second messenger which increases intracellular Ca2+ by mobilizing internal Ca2+ stores, a hypothesis which has been substantiated by studies with chemically permeabilized cells3–13 and with isolated microsomal membrane fractions11,12–16. But the possibility that Ins(1,4,5)P3 could induce in intact cells an influx of external Ca2+ through transmembrane channels, originally hypothesized by Michell in 197517, has never been directly tested. We report here single-channel recordings of an Ins(1,4,5)P3-activated conductance in excised patches of T-lymphocyte plasma membrane. The Ins(1,4,5)P3-activated transmembrane channel appears to be identical to the recently described mitogen-regulated, voltage-insensitive Ca2+ permeable channel involved in T-cell activation18. We suggest that Ins(1,4,5)P3 acts as the second messenger mediating transmembrane Ca2+ influx through specific Ca2+-permeable channels in mitogen-stimulated T-cell activation.
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DOI：
10.1016/s0021-9258(17)42556-7
发表时间：
1984-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Albert;A. Tashjian
通讯作者：
P. Albert;A. Tashjian
影响因子：
64.8
作者：
DECOURSEY, TE;CHANDY, KG;CAHALAN, MD
通讯作者：
CAHALAN, MD
肌醇 1,4,5-三磷酸。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Joseph,SK;Thomas,AP;Williams,RJ;Irvine,RF;Williamson,JR
通讯作者：
Williamson,JR
人类 T 细胞激活的抗原特异性 (T3-Ti) 和替代 (T11) 途径的钙依赖性。
DOI：
10.1073/pnas.81.21.6836
发表时间：
1984
影响因子：
11.1
作者：
Weiss,MJ;Daley,JF;Hodgdon,JC;Reinherz,EL
通讯作者：
Reinherz,EL
肌醇三磷酸介导大鼠促乳腺垂体细胞中非线粒体钙的促甲状腺素释放激素动员。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Gershengorn,MC;Geras,E;Purrello,VS;Rebecchi,MJ
通讯作者：
Rebecchi,MJ