Artificially Created Reentry Circuit by Laser Irradiation Causes Atrial Tachycardia to Persist in Murine Atria
Artificially Created Reentry Circuit by Laser Irradiation Causes Atrial Tachycardia to Persist in Murine Atria
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DOI：
10.1253/circj.cj-20-1286
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发表时间：
2022-02-01
影响因子：
3.3
通讯作者：
Adachi, Takeshi
中科院分区：
文献类型：
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作者：
Horii, Shunpei;Yada, Hirotaka;Adachi, Takeshi
背景：人类的心房颤动（AF）是从阵发性逐渐进展为持续性的。为了阐明所涉及的机制，尝试创建人工心房基质以在小鼠中持续发生房颤。方法和结果：本研究使用野生型（WT）小鼠，但很难在它们中诱导房颤。本研究提出了一种从左心室（LV）顺行灌注以扩大双心房以进行人工心房改造的新方法。在该方法下通过突发起搏诱发短时房颤。光学映射分析揭示了短时自动对焦后非持续焦点类型和蜿蜒的螺旋折返。通过激光照射添加微小的人造基质（直径约 1.2 毫米），以形成关键的房性心律失常基质。在非激光组（n=8）、圆形激光组（n=8）和楔形凹痕激光组（n=8）中进行突发起搏。我们将 AF 和房性心动过速 (AT) 定义为房性心律失常 (AA)。持久 AA 定义为持续 >= 30 分钟。在每组的 0/8、0/8 和 6/8 (75%) 小鼠中观察到持久的 AA。光学映射分析表明，该机制是在照射边缘周围有一个固定转子的 AT。结论：遗憾的是，这项研究未能重现持续性 AF，但成功地创造了一种导致 WT 小鼠持续性 AT 的心律失常基质。
Background: There is a gradual progression from paroxysmal to persistent atrial fibrillation (AF) in humans. To elucidate the mechanism involved, the creation of an artificial atrial substrate to persist AF in mice was attempted.Methods and Results: This study used wild type (WT) mice, but it is difficult to induce AF in them. A novel antegrade perfusion method from the left ventricle (LV) to enlarge both atria for artificial atrial modification was proposed in this study. Short duration AF was induced by burst pacing under this method. Optical mapping analysis revealed non-sustained focal type and meandering spiral reentrants after short duration AF. A tiny artificial substrate (similar to 1.2 mm in diameter) was added in by laser irradiation to create a critical atrial arrhythmogenic substrate. Burst pacing was performed in a non-laser group (n=8), a circular-shape laser group (n=8), and a wedge-shaped dent laser group (n=8). We defined AF and atrial tachycardia (AT) as atrial arrhythmia (AA). Long-lasting AA was defined as lasting for >= 30 min. Long-lasting AA was observed in 0/8, 0/8, and 6/8 (75%) mice in each group. Optical mapping analysis revealed that the mechanism was AT with a stationary rotor around the irradiated margin.Conclusions: Regrettably, this study failed to reproduce persistent AF, but succeeded in creating an arrhythmic substrate that causes sustained AT in WT mice.