Levels of interleukin 1 beta in tissue from sites of active periodontal disease.
Levels of interleukin 1 beta in tissue from sites of active periodontal disease.
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DOI：
10.1111/j.1600-051x.1991.tb00088.x
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发表时间：
1991-08
影响因子：
6.7
通讯作者：
Philip Stashenko;P. Fujiyoshi;M. Obernesser;L. Prostak;A. Haffajee;S. Socransky
中科院分区：
文献类型：
--
作者：
Philip Stashenko;P. Fujiyoshi;M. Obernesser;L. Prostak;A. Haffajee;S. Socransky
白细胞介素1 β是一种有效的骨吸收细胞因子，其还通过刺激前列腺素产生以及诱导胶原酶和其他蛋白酶来介导软组织破坏。这些活性表明IL-1 β在人类牙周炎发病机制中的作用。因此，在从12名患有破坏性成人牙周炎的患者的（1）患病、活动（2）患病、非活动和（3）健康部位获得的组织中测定IL-1 β的水平。疾病活性被定义为大于或等于2.5 mm的附着损失，如通过连续探测和耐受性方法所确定的。从手术中获取的组织活检组织匀浆中提取IL-1 β，并通过ELISA定量其水平。发现IL-1 β存在于大多数患者组织样本中，水平范围为0-82 ng/ml。疾病活动部位的IL-1 β水平高于非活动部位和健康部位（p <0.05）。将脱落的非活动部位分为2组，即脱落少量附着的部位（0.5-2.0 mm，恶化部位）和未显示变化或附着增加的部位（稳定部位）。稳定病变部位的IL-1 β水平与健康部位相当，与活动部位显著不同（p <0.02）。恶化部位的IL-1 β水平介于稳定部位和活性部位的水平之间。在IL-1 β水平大于25 ng/ml（p <0.01）的部位，疾病活动性的检测更频繁。（250字处删节）
Interleukin 1 beta is a potent bone resorptive cytokine which also mediates soft tissue destruction through the stimulation of prostaglandin production, and the induction of collagenase and other proteases. This constellation of activities suggests a role for IL-1 beta in the pathogenesis of human periodontitis. Levels of IL-1 beta were therefore determined in tissue obtained from (1) diseased, active (2) diseased, inactive and (3) healthy sites from 12 patients with destructive adult periodontitis. Disease activity was defined as attachment loss of greater than or equal to 2.5 mm, as determined by sequential probing and the tolerance method. IL-1 beta was extracted from homogenates of tissue biopsies taken at surgery, and levels were quantified by ELISA. IL-1 beta was found to be present in most patient tissue samples, with levels ranging from 0-82 ng/ml. Disease active sites had higher IL-1 beta levels (p less than 0.05) than inactive and healthy sites. Diseased inactive sites were divided into 2 groups, those losing small amounts of attachment (0.5-2.0 mm, worsening sites) and those which showed no change or attachment gain (stable sites). Stable diseased sites had IL-1 beta levels which were comparable to those found in healthy sites, and which were significantly different from active sites (p less than 0.02). Worsening sites had IL-1 beta levels intermediate between the levels in stable and active sites. Detection of disease activity occurred more frequently at sites with IL-1 beta levels greater than 25 ng/ml (p less than 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)