Functional analysis of metal tolerance proteins isolated from Zn/Cd hyperaccumulating ecotype and non‐hyperaccumulating ecotype of Sedum alfredii Hance
Functional analysis of metal tolerance proteins isolated from Zn/Cd hyperaccumulating ecotype and non‐hyperaccumulating ecotype of Sedum alfredii Hance
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DOI：
10.1016/j.febslet.2011.07.013
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发表时间：
2011-08
期刊：
影响因子：
3.5
通讯作者：
M. Zhang;T. Senoura;Xiaoe Yang;N. Nishizawa
中科院分区：
文献类型：
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作者：
M. Zhang;T. Senoura;Xiaoe Yang;N. Nishizawa
The Zn/Cd hyperaccumulating ecotype (HE) of Sedum alfredii Hance can accumulate 24- and 28-fold higher leaf and stem Zn concentrations when compared with the non-hyperaccumulating ecotype (NHE) of Sedum. Heterologous expression of a metal tolerance protein (MTP1) encoding gene from HE plants (SaMTP1) or the homologous gene from NHE plants (SnMTP1) suppressed Zn2+hypersensitivity in the Δzrc1 yeast mutant. In plants, SaMTP1 localized to the tonoplast. Furthermore, MTP1 transcript level in the shoot of HE plants was more than 80-fold higher than that of NHE plants. The transcript level of SaMTP1 in shoot was up-regulated 1-fold by Zn2+while the expression of SnMTP1 was slightly inhibited. These data suggest that SaMTP1 can play an important role in Zn accumulation in HE plants.
