Evidence for three groups of sequence variants of beet necrotic yellow vein virus RNA 5
Evidence for three groups of sequence variants of beet necrotic yellow vein virus RNA 5
复制标题
甜菜坏死黄脉病毒 RNA 5 的三组序列变异的证据
DOI：
10.1007/s007050050553
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发表时间：
1999
影响因子：
2.7
通讯作者：
T. Tamada
中科院分区：
文献类型：
--
作者：
M. Miyanishi;T. Kusume;Minako Saito;T. Tamada
在日本分离的甜菜坏死黄静脉病毒（BNYVV）中，约有一半的分离株含有RNA 5分子，在中国和法国分离的病毒中也检测到RNA 5分子。25株分离株的RNA 5（核苷酸327 ~ 1171）序列差异达8%，RNA 5变异可分为3组：I组包含大部分日本和中国分离株，II组包含2个日本分离株，III组包含4个法国分离株。第一组分离株分为三个小群。在RNA 5的26 kDa编码区，组内核苷酸序列差异最大为1.5%（6个氨基酸变化），组间核苷酸序列差异最大为8.4%（17个氨基酸变化）。RNA 2外壳蛋白基因的比较表明，日本和中国的分离株大部分属于A型菌株，但也有一部分属于B型菌株。法国分离株（P型）与A型亲缘关系密切。在北海道一小部分地区发现了两种病毒和两组RNA 5的混合感染。BNYVV可能通过类似的途径从至少两个来源传入日本和中国。这些结果与其他证据一起表明，RNA 5变体的三组很早以前就从原始种群中分离出来，此后，I组种群进一步分化为三组，这可能与a型菌株而不是B型菌株有关。
About half of Japanese isolates of beet necrotic yellow vein virus (BNYVV) were found to contain RNA 5 molecules, which were also detected in virus isolates from China and France. Sequence comparisons of RNA 5 (nucleotides 327 to 1171) in 25 isolates showed that there are up to 8% sequence differences, and that RNA 5 variants fall into three groups: group I contains most of the Japanese and Chinese isolates, group II two Japanese isolates, and group III four French isolates. The group I isolates fall into three small clusters. In the 26 kDa coding region of RNA 5, there was a maximum of 1.5% nucleotide sequence differences (6 amino acid changes) within the group and 8.4% nucleotide sequence differences (17 amino acid changes) between the groups. Comparisons of the coat protein gene of RNA 2 revealed that most of the Japanese and Chinese isolates belonged to the A type strain, but some isolates were of the B type. The French isolates (P type) were closely related to those of the A type. Mixed infections of the two types of virus and the two groups of RNA 5 were detected in a small area of Hokkaido. BNYVV might have been introduced into Japan and China by a similar route from at least two origins. These results, together with other evidence, suggest that the three groups of RNA 5 variants separated from an original population a long time ago and, thereafter, the group I population diverged further into three clusters, which may have been associated with the A type strain rather than the B type.