Mutagenicity and genotoxicity of dicapthon insecticide
Mutagenicity and genotoxicity of dicapthon insecticide
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DOI：
10.1007/s10616-013-9623-x
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发表时间：
2014-10-01
期刊：
影响因子：
2.2
通讯作者：
Liman, Recep
中科院分区：
文献类型：
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作者：
Liman, Recep
采用鼠伤寒沙门氏菌TA97、TA98、TA100和TA102菌株的致突变试验和体外培养的人外周血淋巴细胞染色体畸变率(CA)、姐妹染色单体互换(SCES)和微核试验(MN)，研究了敌草隆的致突变和遗传毒性。在所有测试体系中，敌草隆都溶解在二甲基亚砜中。0.1、1、10和100 mU g/盘剂量的敌草隆对未加S9混合物的小鼠伤寒沙门氏菌TA 98均有较弱的致突变作用。用25、50、100和200 mU g/m L四种实验浓度敌草隆处理人外周血淋巴细胞24和48 h，在24和48 h，敌草隆只在100 mU g/m L时才能增加姐妹染色单体交换频率。与未处理组和溶剂对照组比较，敌百虫各剂量组均能显著降低细胞分裂指数，100和200mgm L剂量组细胞分裂指数均显著降低，显示出显著的细胞毒作用。这些值也以剂量依赖的方式减少。
Mutagenic and genotoxic effects of dicapthon were investigated by using the bacterial reverse mutation assay in Salmonella typhimurium TA97, TA98, TA100 and TA102 strains with or without metabolic activation system (S9 mix), and chromosome aberrations (CAs), sister chromatid exchanges (SCEs), and micronucleus (MN) tests in human peripheral blood lymphocytes in vitro. Dicapthon was dissolved in dimethyl sulfoxide for all test systems. 0.1, 1, 10 and 100 mu g/plate doses of dicapthon were found to be weakly mutagenic on S. typhimurium TA 98 without S9 mix. The human peripheral lymphocytes were treated with four experimental concentrations of dicapthon (25, 50, 100, and 200 mu g/mL) for 24 and 48 h. Dicapthon increased the frequency of SCE only at the 100 mu g/mL concentration for the 24 and 48 h applications. Dicapthon also induced abnormal cell frequency, CA/cell ratio and frequency of MN dose dependently for 24 and 48 h. Dicapthon showed a statistically significant cytotoxic effect by decreasing the mitotic index in all concentrations and a cytostatic effect by decreasing nuclear division index in 100 and 200 mu g/mL concentrations for both treatment periods when compared with both untreated and solvent controls. These values decreased also in a dose dependent manner.