Copper and iron‐induced oxidative damage in non‐tumor bearing LEC rats
Copper and iron‐induced oxidative damage in non‐tumor bearing LEC rats
复制标题
铜和铁对非荷瘤 LEC 大鼠诱导的氧化损伤
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
S. Okada
中科院分区：
文献类型：
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作者：
Yuxiang Ma;Daxian Zhang;T. Kawabata;T. Kiriu;S. Toyokuni;K. Uchida;S. Okada
本文研究了非荷瘤Long-Evans Cinnamon(LEC)大鼠(平均年龄17个月)肝脏中铜、铁的状况。还研究了使用螯合剂氨三乙酸(NTA可螯合的游离铜和铁)直接定量松散结合的铜和铁的方法。除总铜、铁含量外，LEC大鼠NTA螯合游离铜水平也高于LEA大鼠(P<；0.05)。但两组大鼠的游离铁水平无显著差异(P>；0.05)。LEC大鼠的硫代巴比妥酸反应物质的形成高于LEA大鼠(P<；0.01)。4-羟基-2-壬烯醛(HNE)修饰的蛋白质在LEC大鼠肝脏中也有明显的表达。在LEC氧化损伤过程中产生最重要作用的铜和铁无法区分。尽管LEC大鼠体内可致自由基损伤的游离铜增加，但实验LEC大鼠未见肿瘤和癌前病变。因此，人们推测自由铁的高度是另一个重要因素。铜和铁都是体内重要的翻译金属，可能参与DNA损伤和致癌基因的诱导
The copper and Iron status in the liver of non‐tumor bearing Long‐Evans Cinnamon (LEC) rats (average age 17 months) was investigated. A direct quantitation of loosely‐bound copper and iron was also investigated by using a chelating agent, nitrilotriacetic acid (NTA‐chelatable free copper and iron). Besides the total copper and iron contents, the level of NTA‐chelatable free copper was also higher in LEC rats than In LEA rats (P<0.05). But for the free iron level there was no signiflcant difference between the two rat groups (P>0.05). The formation of thiobarbituric acid‐reactive substances was higher In LEC rats than In LEA rats (P<0.01). The 4–hydroxy‐2–nonenal (HNE)‐modified proteins were also clearly demonstrated in LEC rat liver. The copper and iron which produced the most important effect In the process of oxidative damage in LEC rats could not be distinguished. Even though free copper, which could induce free radical injuries, was increased in LEC rats, neither tumor‐induction nor preneo‐plastic lesions in the experimental LEC rats were observed. Therefore it is speculated that the elevation of a free iron is another important factor. Copper and iron, both important translation metals In the body, may participate In the Induction of DNA damage and oncogenesls