Isolation and sequence analysis of the gene for translation elongation factor 3 from Candida albicans.
Isolation and sequence analysis of the gene for translation elongation factor 3 from Candida albicans.
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DOI：
10.1093/nar/20.7.1705
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发表时间：
1992-04
影响因子：
14.9
通讯作者：
K. K. Myers-K.;W. Fonzi;P. Sypherd
中科院分区：
文献类型：
--
作者：
K. K. Myers-K.;W. Fonzi;P. Sypherd
延伸因子3（EF-3）是真菌翻译系统中一个独特的和必不可少的组成部分。CEF-3基因编码的延伸因子3已经从二型真菌白色念珠菌中分离出来。利用含有酿酒酵母EF-3基因编码区的异源基因探针筛选3个白念珠菌基因组DNA文库。测定四个部分克隆的核苷酸序列，并将其组合为3，671个碱基对（bp）的全长。该基因的开放阅读框为3,147 bp，编码1,049个氨基酸，分子量为116,739道尔顿。在使用CEF-3基因探针的白色念珠菌总RNA的北方印迹杂交中观察到3，400个核苷酸的转录物。单基因座CEF-3基因定位于基因组中的第5号染色体。CEF-3与YEF-3推导的氨基酸序列的同源性为77.6%。身份当比较两种蛋白质的羧基末端部分时，发现更高程度的同一性，86.5%。在核苷酸水平上，两个基因的编码区的比较显示79%的同一性，而上游和下游区域分别显示46%和40%的同一性。
Elongation factor 3 (EF-3) is a unique and essential component of the translational system in fungi. The gene, CEF-3, encoding elongation factor 3 has been isolated from the dimorphic fungus Candida albicans. A heterologous gene probe containing the coding region of the EF-3 gene from Saccharomyces cerevisiae (YEF-3) was used to screen three Candida albicans genomic DNA libraries. The nucleotide sequences of four partial clones were determined and combined for a full-length of 3,671 base pairs (bp). A continuous open reading frame (ORF) of 3,147 bp encoding a predicted protein of 1,049 amino acids and Mr of 116,739 daltons has been identified. A transcript of 3,400 nucleotides is seen in Northern blot hybridization of Candida albicans total RNA using a CEF-3 gene probe. The single locus CEF-3 gene maps to chromosome 5 in the genome. Comparison of the deduced amino acid sequences of CEF-3 and YEF-3 shows 77.6%. identity. A higher degree of identity, 86.5%, is found when comparing the carboxy-terminal portions of the two proteins. At the nucleotide level, comparison of the coding regions of the two genes exhibit 79% identity while the upstream and downstream regions show 46% and 40% identity, respectively.