Transgenic tobacco plants expressing a coat protein gene of tobacco mosaic virus are resistant to some other tobamoviruses.
Transgenic tobacco plants expressing a coat protein gene of tobacco mosaic virus are resistant to some other tobamoviruses.
复制标题
表达烟草花叶病毒外壳蛋白基因的转基因烟草植物对一些其他烟草病毒具有抗性。
DOI：
10.1094/mpmi-3-247
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Beachy,RN
中科院分区：
文献类型：
--
作者：
Nejidat,A;Beachy,RN
MATERIALS AND METHODSPlant material. Tobacco (Nicotiana tabacum L.) cultivars Xanthi and Xanthi-nc were used as systemic and local lesion hosts, respectively. Transgenic plants of Xanthi tobacco expressing the TMV CP gene (CP") are progeny of lines3646 and 3404 (Powell Abel et al. 1986) and Xanthi-nc line 748 (Nelson et al. 1987). CPT (nontransformed) Xanthi and Xanthi-nc were used as controls. Plants were grown in a greenhouse under natural lighting conditions, supplemented in the winter with artificial light to provide 14-hr days. Plants at 25–27 days after seed germination (systemic host) or 35–40 days after germination (local lesion host) were used in all experiments. Sources and purification of viruses and RNA. Strain L of ToMV was obtained from M. Zaitlin (Cornell University, Ithaca, NY). Tobacco mild green mosaic virus (TMGMV) or the U2 strain of TMV (Wetter 1986); Odontoglossum ringspot virus (ORSV), also known as the orchid strain of TMV (Edwardson and Zettler 1986); and ribgrass mosaic virus (RMV) were obtained from the American Type Culture Collection (ATCC PV228, PV318, and PV229, respectively.) Pepper mild mottle virus (PMMV) was kindly supplied by BL Subba Rao (Chemtex, Port Arthur, TX). All viruses were passed through a single local lesion host and subsequently propagated in an appropriate systemic host as indicated in Table 1. All viruses were purified as described by Asselin and Zaitlin (1978). RNA was isolated as described by Bruening et al.(1976).
