Paramyxoviral and reoviral infections of iguanas on Honduran islands
Paramyxoviral and reoviral infections of iguanas on Honduran islands
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DOI：
10.7589/0090-3558-34.1.33
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发表时间：
1998-01-01
影响因子：
1.3
通讯作者：
Kaleta, EF
中科院分区：
文献类型：
--
作者：
Gravendyck, M;Ammermann, P;Kaleta, EF
35只自由放养的健康刺尾鬣蜥（Ctenosaura bakeri 31只，C. similis）和14只绿色鬣蜥（Iguanaguanagulolopha）被捕获并圈养2天。采集所有动物的血液，并评价其血清中抗爬行动物呼肠孤病毒、爬行动物副粘病毒和禽类副粘病毒-1（PMV-1）的抗体滴度。还收集了泄殖腔和咽拭子，并通过在鸡胚成纤维细胞（CEF）和Terrapene心脏细胞（TH-1）上孵育来检查病毒含量。在CEF和TH-1上，从咽拭子和泄殖腔拭子中未分离到病毒。通过病毒中和试验，49份血清样品中有23份（47%）检测为爬行动物呼肠孤病毒阳性。通过病毒中和试验，49个样本中有20个（41%）具有针对一种爬行动物PMV分离株的抗体，通过血凝抑制试验，34个样本中有3个（9%）具有针对一种爬行动物PMV分离株的抗体。未检测到针对其他爬行动物来源的PMV分离株或针对禽类PMV-1的抗体。这是第一个描述的血清抗体对爬行动物呼肠孤病毒和PMV在野生鬣蜥。
Thirty-five free-ranging healthy spiny-tailed iguanas (31 Ctenosaura bakeri, 4 C. similis) and 14 green iguanas (Iguana iguana rhinolopha) were caught and held in captivity for 2 days. Blood was collected from all animals and their sera were evaluated for antibody titres against reptilian reoviruses, reptilian paramyxoviruses, and avian paramyxovirus-1 (PMV-1). Cloacal and pharyngeal swabs also were collected and examined for viral content by incubation on chicken embryo fibroblasts (CEF) and terrapene heart cells (TH-1). No virus was isolated from the pharyngeal and cloacal swabs on CEF and TH-1. Twenty-three (47%) of 49 sera samples tested positive for reptilian reoviruses by virus neutralization tests. Twenty (41%) of 49 samples had antibodies against one reptilian PMV isolate by virus neutralization tests and 3 (9%) of 34 by hemagglutination inhibition tests. No antibodies were detected against the other PMV isolate of reptilian origin nor against avian PMV-1. This is the first description of serum antibodies against reptilian reoviruses and PMV in wild iguanas.