Thyroid fine needle aspiration:: the morphological features on ThinPrep® slide preparations.: Eighty cases with histological control
Thyroid fine needle aspiration:: the morphological features on ThinPrep® slide preparations.: Eighty cases with histological control
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DOI：
10.1046/j.0956-5507.2003.00098.x
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发表时间：
2003-12-01
期刊：
影响因子：
1.3
通讯作者：
Guillausseau, PJ
中科院分区：
文献类型：
--
作者：
Cochand-Priollet, B;Prat, JJ;Guillausseau, PJ
本研究有几个目的：确定可能被酒精固定改变的甲状腺细胞的细胞形态学特征；优化ThinPrep((R)) （TP; Cytyc Inc., Bexborough， MA， USA）载玻片上的mayg - grunwald - giemsa （MGG）染色，并比较液基法制备的载玻片与常规技术制备的载玻片的诊断准确性。本研究包括120例超声引导下甲状腺细针穿刺（FNA）和55例手术切除甲状腺标本的FNA。80例有组织学对照。在第一组120个FNAs中，使用分裂样本技术进行TP。进行了三次筛查：首先，对常规涂片（CS）和TP进行单独筛查，第二次筛查将TP上观察到的细胞与组织学对照进行比较，第三次筛查评估先前定义的诊断标准。TP 27例（22%）诊断不满意，CS 10例（8%）诊断不满意。TP不满意病例的高比率可能是由于使用了分裂样本技术。CS的敏感性为94%，TP的敏感性为81%。CS和TP的特异性分别为67%和60%。两种方法均未发现2例隐匿性乳头状癌。对于MGG染色，改良后的TP染色技术的质量与标准方法相同。相反，TP确实诱导了不常见的形态学特征。在本研究中，CS的敏感性和特异性水平高于TP；这种差异可能是由于用于TP的甲醇固定剂引起了一些细胞学改变，特别是在嗜瘤性肿瘤和淋巴细胞性甲状腺炎中。
This study had several purposes: to define cytomorphological features of thyroid cells that might be modified by alcohol fixation; to optimize May-Grunwald-Giemsa (MGG) staining on ThinPrep((R)) (TP; Cytyc Inc., Bexborough, MA, USA) slides and to compare the diagnostic accuracy of slides prepared by a liquid-based method with those obtained by conventional technique. This study included 120 cases of ultrasound-guided fine needle aspiration (FNA) of the thyroid and 55 FNAs performed on surgically resected thyroid specimens. Histological control was available in 80 cases. In the first group of 120 FNAs, a split-sample technique was used for the TP. Three screenings were performed: first, an individual screening of the conventional smears (CS) and of the TP, a second screening to compare cells observed on the TP with the histological control and a third screening to assess the previously defined diagnostic criteria. Twenty-seven TP cases (22%) were considered unsatisfactory for diagnosis compared with 10 in CS (8%). The high rate of unsatisfactory cases with TP is likely to be due to the use of the split-sample technique. The sensitivity was 94% for CS and 81% for TP. The specificity was 67% and 60% for CS and TP, respectively. Two occult papillary carcinomas were missed by both methods. As for the MGG staining, the modified technique used for TP resulted in the same quality as the standard procedure. Conversely, TP did however induce uncommon morphological features. In this study, sensitivity and specificity levels are higher for CS than for TP; the difference may be explained by the fact that the methanol fixative used for TP induces some cytological alterations, especially in oncocytic tumours and lymphocytic thyroiditis.