Evaluation of Betulin and Betulinic Acid Content in Birch Bark from Different Forestry Areas of Western Carpathians
Evaluation of Betulin and Betulinic Acid Content in Birch Bark from Different Forestry Areas of Western Carpathians
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西喀尔巴阡不同林区桦树皮中桦木醇和桦木酸含量评价
DOI：
10.15835/nbha4027967
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
C. Socaciu
中科院分区：
文献类型：
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作者：
L. Holonec;F. Ranga;D. Crainic;A. Truta;C. Socaciu
为了评估桦树皮中桦木醇和桦木酸的含量，从西喀尔巴阡山脉，特兰西瓦尼亚（罗马尼亚）的森林，与桦树的位置，它被记录在每种类型的样品的特定位置的地理和植被条件。采集了两组桦树皮（共10个样品），分别来自两个地区，桦树自然生长的牧场表面（样品1至5）和另一组来自以松树为主的混交林（样品6至10）。通过高效液相色谱-紫外检测器对白桦脂醇和白桦脂酸进行了鉴别和定量。在第一个区域，发现白桦脂醇树皮的平均浓度为126.85 ± 12.56 mg/g，而酸白桦脂酸的平均值为12.78 ± 1.26 mg/g树皮。在第二个区域，我们发现树皮中桦木醇的平均浓度为89.84 ± 8.43 mg/g，而酸性桦木酸的平均值为10.60 ± 0.97 mg/g。从统计学上看，两个区域之间存在显著差异，第一个区域更富含这些分子。一般来说，白桦脂酸相对于白桦脂醇的百分比约为11.23%，在第二区域较高，即使两种分子的绝对浓度较低。这些数据是有用的指标，表明了在跨西尔瓦尼亚地区发现的桦树皮来源提供的潜力，以获得富含桦木醇和桦木酸的提取物。
In order to evaluate the content of betulin and betulinic acids in Birch barks originating from Western Carpathians, Transylvania (Romania) forests, in relation with the location of birch trees, it was recorded the geographic and vegetation conditions at the specific locations of each type of sample. Two groups of birch tree bark (totally 10 samples) were collected, from two regions, pasture surfaces where birch trees appeared spontaneously (samples 1 to 5) and another group from forests of combined trees, including mainly pine trees (samples 6 to 10). The identification and quantitation of betulin and betulinic acid were made by High Performance Liquid Chromatography with UV detection. In the first region it has been found mean concentrations of 126.85 ± 12.56 mg/g betulin bark while acid betulinic had average values of 12.78 ± 1.26 mg/g bark. In the second region we found mean concentrations of 89.84 ± 8.43 mg/g betulin of bark while acid betulinic had average values of 10.60 ± 0.97 mg/g bark. Statistically, significant differences were noticed between the two regions, the first region being richer in these molecules. Generally, the percentage of betulinic acid was around 11.23% against betulin, higher in the second region, even the absolute concentrations were lower for both molecules. These data are useful indicators of the potential offered by birch bark sources found in Transylvania region, to obtain extracts enriched in betulin and betulinic acid.