REMOVAL OF ENDOTOXIN FROM PROTEIN SOLUTIONS BY PHASE-SEPARATION USING TRITON X-114
REMOVAL OF ENDOTOXIN FROM PROTEIN SOLUTIONS BY PHASE-SEPARATION USING TRITON X-114
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DOI：
10.1016/0022-1759(90)90029-u
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发表时间：
1990-09-14
影响因子：
2.2
通讯作者：
PABST, MJ
中科院分区：
文献类型：
--
作者：
AIDA, Y;PABST, MJ
蛋白质溶液的内毒素污染减少相分离技术，使用清洁剂，Triton X-114。在冰上用Triton X-114处理含有内毒素的蛋白质溶液。然后将溶液加热至37 ℃。C，于是形成了两相。离心沉淀含有内毒素的Triton X-114相。相分离的第一个循环产生了1000倍的细胞色素c，过氧化氢酶和白蛋白的污染制剂的内毒素减少。通过进一步的相分离循环可以完全去除内毒素。每个相分离循环仅导致2%的蛋白质损失，并且可以在15 min内完成。少量的去污剂（0.018%）比蛋白质溶液中的残留物可以通过凝胶过滤或吸附去除。经此程序处理的蛋白质保留了正常功能。这种相分离技术提供了一种从蛋白质溶液中去除内毒素的快速而温和的方法。
Endotoxin contamination of protein solutions was reduced by a phase separation technique using the detergent, triton X-114. Protein solutions containing endotoxin were treated with Triton X-114 on ice. The solution was then warmed to 37.degree. C, whereupon two phases formed. the Triton X-114 phase, containing the endotoxin, was precipitated by centrifugation. The first cycle of phase separation produced a 1000-fold reduction of endotoxin from contaminated preparations of cytochrome c, catalase and albumin. Complete removal of endotoxin could be achieved by further cycles of phase separation. Each cycle of phase separation resulted in only a 2% loss of protein, and could be completed within 15 min. The small amount of detergent (0.018%) than persisted in protein solution could be removed by gel filtration or absorption. Proteins treated by this procedure retained normal functions. This phase separation technique provides a rapid and gentle method for removing endotoxin from protein solutions.