In Vitro and In Vivo Biofilm Wound Models and Their Application
In Vitro and In Vivo Biofilm Wound Models and Their Application
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DOI：
10.1007/5584_2015_5002
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发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Coenye, Tom
中科院分区：
文献类型：
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作者：
Brackman, Gilles;Coenye, Tom
慢性伤口是在正常伤口愈合的一个或多个阶段中滞留的伤口。据估计，发达国家1- 2%的人口将在其一生中经历慢性伤口，并且鉴于世界人口的增长、年龄、体重指数和相关疾病（如糖尿病和心血管疾病）的增加，预计这一数字将增加。虽然有几个因素有助于伤口愈合，但细菌生物膜的存在显著影响伤口治疗的愈合和成功。这表明伤口护理疗法应针对慢性伤口内的靶向生物膜。尽管如此，生物膜在慢性伤口发病机制中的作用以及伤口护理疗法对伤口内生物膜的影响尚未得到很好的理解。为了解决这些问题，适当的生物膜模型是必要的。为此，已经开发了几种模拟在生物膜感染的慢性伤口中观察到的条件的模型系统。在这篇综述中，我们提出了这些不同的体外和体内生物膜伤口模型系统的概述，并讨论其优点和缺点。
Chronic wounds are wounds which are detained in one or more phases of normal wound healing. It is estimated that 1-2 % of the population of developed countries will experience a chronic wound during their lifetime and this number is expected to increase given the growing world population, increase in age, body mass index and associated diseases such as diabetes and cardiovascular diseases. Although several factors contribute to wound healing, presence of bacterial biofilms significantly affects healing and success of wound treatment. This indicates that wound-care therapies should be directed towards targeting biofilms within chronic wounds. Despite this, the role of biofilms in chronic wound pathogenesis and the effect of wound-care therapies against biofilms within wounds are not well understood. In order to address these issues, appropriate biofilm models are necessary. To this end, several model systems mimicking the conditions observed in a biofilm infected chronic wound have been developed. In this review we present an overview of these different in vitro and in vivo biofilm wound model systems and discuss their advantages and disadvantages.