Catalytic activity of Cerrena unicolor laccase in aqueous solutions of water-miscible organic solvents -: Experimental and numerical description
Catalytic activity of Cerrena unicolor laccase in aqueous solutions of water-miscible organic solvents -: Experimental and numerical description
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DOI：
10.1016/j.molcatb.2006.08.005
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发表时间：
2007-02-01
影响因子：
--
通讯作者：
Jarosz-Wilkolazka, A.
中科院分区：
文献类型：
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作者：
Rodakiewicz-Nowak, J.;Jarosz-Wilkolazka, A.
研究了在乙醇、丙酮和二甲基亚砜（DMSO）水溶液中，漆酶对乌柳甲津（SGZ）氧化反应的稳定性和催化活性。此外，还研究了邻苯二酚（CAT）和2，6-二甲氧基苯酚（DMOP）在乙醇和DMSO溶液中的氧化反应。据观察，在3 M的溶液中的研究溶剂CUL是实际上稳定的30分钟的实验，这表明其良好的适用性，在水混溶性有机溶剂的溶液。此外，据观察，测试的有机溶剂对不同底物的最大氧化速率值（V-max）的影响不依赖于底物。在研究的浓度范围内（高达8 M），乙醇不影响Vmax值，DMSO的存在使其以与研究底物大致相同的速率降低。在SGZ作为底物的情况下，溶剂对V-max值的影响按乙醇<丙酮< DMSO的顺序增加，而在K-M的情况下，溶剂对V-max值的影响按DMSO <丙酮<乙醇的顺序增加。有机溶剂的含量和所观察到的动力学参数之间的实验关系进行了数值描述，使用溶剂浓度的指数关系，在第一近似取代反应物的活度系数的变化。拟合的指数系数与其他真菌漆酶的观察值进行了观察和讨论。(c)2006 Elsevier B. V.保留所有权利。
The stability and catalytic activity during oxidation of syringaidazine (SGZ) by laccase from Cerrena unicolor (CUL) was studied in aqueous solutions of ethanol, acetone and dimethyl sulfoxide (DMSO). Additional, the oxidation of other two substrates, catechol (CAT) and 2,6-dimethoxyphenol (DMOP), was studied in ethanol and in DMSO solutions, respectively. It was observed that in 3 M solutions of the studied solvents CUL was practically stable for 30 min of the experiments, which show its good applicability in solutions of water-miscible organic solvents. Moreover, it was observed that the effect of the tested organic solvents on the maximum rate of oxidation values (V-max) of different substrates was not dependent on the substrate. Within the studied range of concentrations (up to 8 M) ethanol did not affect V-max values and the presence of DMSO reduced it at roughly the same rate for the studied substrates. In the case of SGZ as a substrate, the effect of the solvent on V-max values increased in the order ethanol < acetone < DMSO and in the case of K-M in the order DMSO < acetone < ethanol. The experimental relationships between the content of organic solvents and the observed kinetic parameters were numerically described using the exponential relationships on the solvent concentrations, in the first approximations replacing the variability of the activity coefficients of the reactants. The fitted exponential coefficients were observed with the values observed for other fungal laccases and discussed. (c) 2006 Elsevier B.V. All rights reserved.