Opto-μECoG Array: A Hybrid Neural Interface With Transparent μECoG Electrode Array and Integrated LEDs for Optogenetics
Opto-μECoG Array: A Hybrid Neural Interface With Transparent μECoG Electrode Array and Integrated LEDs for Optogenetics
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DOI：
10.1109/tbcas.2013.2282318
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发表时间：
2013-10-01
影响因子：
5.1
通讯作者：
Li, Wen
中科院分区：
文献类型：
--
作者：
Kwon, Ki Yong;Sirowatka, Brenton;Li, Wen
皮层电图（ECoG）记录，从放置在皮层表面的电极，已成功地实现了脑机接口（BMI）的控制。光遗传学，直接光学刺激脑组织中的神经元基因修饰表达通道视紫红质-2（ChR 2），能够以亚毫秒的时间精度靶向特定类型的神经元。在这项工作中，我们开发了一种BMI设备，称为Opto-mu ECoG阵列，它结合了ECoG记录和基于光遗传学的刺激，以实现与神经元的多通道双向相互作用。Opto-mu ECoG阵列包括两个子阵列，每个子阵列包含4 x 4分布的微硬膜外透明电极（类似于200 μ m直径）和嵌入式发光二极管（LED），用于在2.5 x 2.5 mm（2）足迹上进行光学神经刺激，以匹配大鼠视觉皮层的双侧半球区域。透明电极用氧化铟锡（ITO）制造。聚对二甲苯-C作为主要结构和包装材料，具有灵活性和生物相容性。光学，电气和热特性的制造设备进行了研究，并在体内进行实验，以评估该设备的功效。
Electrocorticogram (ECoG) recordings, taken from electrodes placed on the surface of the cortex, have been successfully implemented for control of brain machine interfaces (BMIs). Optogenetics, direct optical stimulation of neurons in brain tissue genetically modified to express channelrhodopsin-2 (ChR2), enables targeting of specific types of neurons with sub-millisecond temporal precision. In this work, we developed a BMI device, called an Opto-mu ECoG array, which combines ECoG recording and optogenetics-based stimulation to enable multichannel, bi-directional interactions with neurons. The Opto-mu ECoG array comprises two sub-arrays, each containing a 4 x 4 distribution of micro-epidural transparent electrodes (similar to 200 mu m diameter) and embedded light-emitting diodes (LEDs) for optical neural stimulation on a 2.5 x 2.5 mm(2) footprint to match the bilateral hemispherical area of the visual cortex in a rat. The transparent electrodes were fabricated with indium tin oxide (ITO). Parylene-C served as the main structural and packaging material for flexibility and biocompatibility. Optical, electrical, and thermal characteristics of the fabricated device were investigated and in vivo experiments were performed to evaluate the efficacy of the device.