Spatial organization of single mRNPs at different stages of the gene expression pathway
Spatial organization of single mRNPs at different stages of the gene expression pathway
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DOI：
10.1101/237008
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发表时间：
2018-07
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通讯作者：
Srivathsan Adivarahan;Nathan M. Livingston;Beth L. Nicholson;Samir Rahman;Bin Wu;Olivia S. Rissland;Daniel Zenklusen
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文献类型：
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作者：
Srivathsan Adivarahan;Nathan M. Livingston;Beth L. Nicholson;Samir Rahman;Bin Wu;Olivia S. Rissland;Daniel Zenklusen
mRNA 通过与对 mRNA 代谢至关重要的蛋白质结合形成核糖核蛋白复合物 (mRNP)。虽然 mRNP 蛋白质组已得到很好的表征，但人们对 mRNP 的组织知之甚少。使用单分子方法，我们证明 mRNA 构象的变化取决于其细胞定位和翻译状态。与核 mRNP 和 lncRNP 相比，与核糖体的结合使单个 mRNA 解压缩，而它们隔离到应力颗粒中导致压实增加。此外，翻译的 mRNA 很少表现出共定位的 5' 和 3' 末端，这表明 mRNA 要么不以闭环构型翻译，要么 mRNA 环化是瞬时的，这表明稳定的闭环构象并不是所有翻译 mRNA 的普遍状态。一句话总结细胞中的单 mRNA 研究表明，RNA 压缩会根据翻译状态而变化，但 mRNA 不会以闭环构象进行翻译。
mRNAs form ribonucleoprotein complexes (mRNPs) by association with proteins that are crucial for mRNA metabolism. While the mRNP proteome has been well characterized, little is known about mRNP organization. Using a single molecule approach, we show that mRNA conformation changes depending on its cellular localization and translational state. Compared to nuclear mRNPs and lncRNPs, association with ribosomes decompacts individual mRNAs, while their sequestration into stress-granules leads to increased compaction. Moreover, translating mRNAs rarely show co-localizing 5’ and 3’ ends, indicating that mRNAs are either not translated in a closed-loop configuration, or that mRNA circularization is transient, suggesting that a stable closed-loop conformation is not a universal state for all translating mRNAs. One Sentence Summary Single mRNA studies in cells show RNA compaction changes depending on translational state, but mRNAs are not translated in closed-loop conformation.