AMELOGENIN POST-SECRETORY PROCESSING DURING BIOMINERALIZATION IN THE POSTNATAL MOUSE MOLAR TOOTH
AMELOGENIN POST-SECRETORY PROCESSING DURING BIOMINERALIZATION IN THE POSTNATAL MOUSE MOLAR TOOTH
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DOI：
10.1016/0003-9969(91)90101-y
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发表时间：
1991-01-01
影响因子：
3
通讯作者：
SNEAD, ML
中科院分区：
文献类型：
--
作者：
FINCHAM, AG;HU, Y;SNEAD, ML
发育中牙齿的釉原蛋白的一级结构、分子遗传学和生物合成已经建立，但其随后的分泌后加工及其与釉质生物矿化的关系的知识是零碎的。 从出生至15天的小鼠磨牙（M1）中分离牙基质蛋白，并通过SDS-PAGE和免疫化学方法进行分析。 通过HPLC分离和部分纯化的釉原蛋白通过氨基酸分析和SDS-PAGE表征。 在出生时，存在26 kDa的釉原蛋白，在随后的发育阶段产生一系列20-25 kDa的釉原蛋白，表观大小相差约1 kDa。 氨基酸分析表明，所有这些釉原蛋白具有氨基末端TRAP序列，糖基化和磷酸化分析均为阴性。 有人建议，这些分泌后釉原蛋白产生的序列的特定的羧基末端裂解，并观察到的分泌后加工的釉原蛋白的功能与釉质基质的结构和控制微晶发育。
The primary structures, molecular genetics and biosynthesis of the amelogenin protein of the developing tooth are established, but knowledge of their subsequent post-secretory processing and its relation to enamel biomineralization is fragmentary. Preparations of tooth matrix proteins were isolated from molars (M1) of mice from birth to 15 days and analysed by SDS-PAGE and immunochemical methods. Amelogenin proteins, isolated and partially purified by HPLC, were characterized by amino acid analysis and SDS-PAGE. At birth a 26 kDa amelogenin was present that during subsequent developmental stages generated a series of 20-25 kDa amelogenins differing in apparent size by approximately 1 kDa. Amino acid analyses showed that all these amelogenins have amino-terminal TRAP sequences; analyses for both glycosylation and phosphorylation were negative. It is suggested that these post-secretory amelogenins are generated by a sequence of specific carboxy-terminal cleavages, and that the observed post-secretory processing of amelogenin is functionally linked to the structure of the enamel matrix and the control of crystallite development.