Effect of Both Ultraviolet B Irradiation and Histamine Receptor Function on Allergic Responses to an Inhaled Antigen1
Effect of Both Ultraviolet B Irradiation and Histamine Receptor Function on Allergic Responses to an Inhaled Antigen1
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紫外线 B 照射和组胺受体功能对吸入抗原过敏反应的影响1
作者：
Jacqueline P. McGlade;S. Gorman;J. Lenzo;Jamie W. Tan;Takeshi Watanabe;J. Finlay;W. Thomas;P. Hart
皮肤暴露于UVB辐射（290-320 nm）调节免疫系统，大多数研究显示抑制Th 1驱动的免疫应答。本研究采用小鼠过敏性呼吸道炎症模型研究UVB对Th 2相关免疫应答的影响。C57 BL/6、组胺受体-1敲除（H1 RKO）和组胺受体-2敲除（H2 RKO）小鼠在用木瓜蛋白酶（尘螨过敏原Der p 1的半胱氨酸蛋白酶同源物）鼻内致敏前3天，在剃毛的背部皮肤上暴露于单次4 kJ/m2剂量的UVB（最小水肿剂量的两倍）。H1 RKO小鼠在肺引流淋巴结（LDLN）中表现出增强的木瓜蛋白酶特异性炎症反应，而H2 RKO小鼠的反应与C57 BL/6小鼠的反应非常相似。致敏前3天的UVB照射降低了C57 BL/6和H1 RKO小鼠的LDLN细胞体外木瓜蛋白酶特异性增殖，但在激发后24 h的H2 RKO小鼠则没有。UVB的调节作用转移过继转移未分级的LDLN细胞从UVB照射，木瓜蛋白酶致敏的C57 BL/6和H1 RKO供体小鼠在相应的菌株，随后用木瓜蛋白酶致敏和挑战的幼稚受体。此外，UVB暴露抑制木瓜蛋白酶诱导的IL-5和IL-10的生产在体外的LDLN细胞从H1 RKO小鼠，但不从C57 BL/6小鼠或H2 RKO小鼠。本研究的结果表明，通过UVB照射鼻内递送Ag的反应的系统性免疫调节，并暗示H2受体在UVB诱导的引流淋巴结中Ag特异性反应的抑制中的作用。
Exposure of skin to UVB radiation (290–320 nm) modulates the immune system, with most studies showing a suppression of Th1-driven immune responses. This study investigated the effects of UVB on Th2-associated immune responses using a murine model of allergic respiratory inflammation. C57BL/6, histamine receptor-1 knockout (H1RKO), and histamine receptor-2 knockout (H2RKO) mice were exposed to a single 4 kJ/m2 dose of UVB (twice a minimal edemal dose) on shaved dorsal skin 3 days before intranasal sensitization with papain, a cysteine protease homologue of the dust mite allergen Der p 1. H1RKO mice demonstrated enhanced papain-specific inflammatory responses in the lung-draining lymph nodes (LDLNs), whereas the responses of H2RKO mice closely mimicked those of C57BL/6 mice. UVB irradiation 3 days before sensitization reduced in vitro papain-specific proliferation of LDLN cells of C57BL/6 and H1RKO mice but not H2RKO mice 24 h after challenge. The regulatory effect of UVB was transferred by adoptive transfer of unfractionated LDLN cells from UVB-irradiated, papain-sensitized C57BL/6 and H1RKO donor mice in naive recipients of the corresponding strain that were subsequently sensitized and challenged with papain. Additionally, UVB exposure suppressed papain-induced IL-5 and IL-10 production in vitro by LDLN cells from H1RKO mice but not from C57BL/6 mice or H2RKO mice. The results of this study demonstrate systemic immunomodulation of responses to intranasally delivered Ag by UVB irradiation and implicate a role for the H2 receptor in UVB-induced suppression of Ag-specific responses in the draining lymph nodes.
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影响因子：
--
作者：
I. Walters;M. Ozawa;I. Cardinale;P. Gilleaudeau;W. Trepicchio;J. Bliss;J. Krueger
通讯作者：
I. Walters;M. Ozawa;I. Cardinale;P. Gilleaudeau;W. Trepicchio;J. Bliss;J. Krueger
组胺调节人类溶细胞性 T 淋巴细胞的生成。
影响因子：
4.3
作者：
Khan,MM;Keaney,KM;Melmon,KL;Clayberger,C;Krensky,AM
通讯作者：
Krensky,AM
影响因子：
6.5
作者：
Nghiem, DX;Kazimi, N;Ullrich, SE
通讯作者：
Ullrich, SE
哮喘：病理学和病理生理学。
影响因子：
4.6
作者：
Barrios,RobertoJ;Kheradmand,Farrah;Batts,LaKeisha;Corry,DavidB
通讯作者：
Corry,DavidB
自体激素对 T 细胞亚群的药理作用。
DOI：
10.1172/jci111863
发表时间：
1985
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Khan,MM;Sansoni,P;Engleman,EG;Melmon,KL
通讯作者：
Melmon,KL