Membrane activation: Selective vesicle fusion via small molecule recognition
Membrane activation: Selective vesicle fusion via small molecule recognition
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DOI：
10.1021/ja0644576
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发表时间：
2006-11-15
影响因子：
15
通讯作者：
Bong, Dennis
中科院分区：
文献类型：
--
作者：
Gong, Yun;Luo, Yumei;Bong, Dennis
我们在此报告的诱导选择性囊泡融合与生物识别基序不天然相关的脂质双层融合，从而扩大了范围的免疫引导的膜活化。我们的系统采用万古霉素糖肽，偶联到抗菌肽爪蟾抗菌肽，和D-丙氨酸-D-丙氨酸-OH二肽偶联到磷脂衍生物，作为表面结合的融合剂。融合的特点是动态光散射和FRET实验与脂质结合的荧光团。我们在这里已经证明，适当设计的膜锚定分子识别基序具有激活特定膜合并的仿生能力;这一原理与靶向化学递送和纳米级区室化化学中的目标产生了共鸣。
We report herein the induction of selective vesicle fusion with biological recognition motifs not natively associated with lipid bilayer fusion, thus broadening the scope of recognition-guided membrane activation. Our system employs vancomycin glycopeptide, coupled to the antimicrobial peptide magainin, and D-Ala-D-Ala-OH dipeptide coupled to a phospholipid derivative, as surface-bound fusogens. Fusion was characterized by dynamic light scattering and FRET experiments with lipid bound fluorophores. We have demonstrated here that appropriately designed membrane anchored molecular recognition motifs have the biomimetic ability to activate specific membrane mergers; this principle has resonance with goals in targeted chemical delivery and nanoscale compartmentalized chemistry.