THE CYTOTOXIC PROTEIN YOPE OF YERSINIA OBSTRUCTS THE PRIMARY HOST DEFENSE
THE CYTOTOXIC PROTEIN YOPE OF YERSINIA OBSTRUCTS THE PRIMARY HOST DEFENSE
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DOI：
10.1111/j.1365-2958.1990.tb00635.x
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发表时间：
1990-04-01
影响因子：
3.6
通讯作者：
WOLFWATZ, H
中科院分区：
文献类型：
--
作者：
ROSQVIST, R;FORSBERG, A;WOLFWATZ, H
先前已表明，假结核耶尔森氏菌的质粒编码的 YopE 蛋白是毒力决定簇。在这项研究中，HeLa细胞、巨噬细胞和小鼠被用作不同的模型系统，以确定YopE在毒力过程中的实际作用。 YopE 蛋白介导 HeLa 细胞汇合层上的细胞毒性反应。这种活性的先决条件是病原体与细胞表面结合。 YopE 还会诱导小鼠巨噬细胞产生细胞毒性反应，从而影响病原体抵抗吞噬作用的能力。表达 YopE 能力缺陷的细菌突变体在口服或腹膜内感染后无毒力，但在静脉注射后有毒力。根据这些结果，我们提出了 YopE 在耶尔森氏菌毒力过程中的作用。
It has previously been shown that the plasmid-encoded YopE protein of Yersinia pseudotuberculosis is a virulence determinant. In this study, HeLa cells, macrophages and mice were used as different model systems to determine the actual role of YopE in the virulence process. The YopE protein mediates a cytotoxic response on a confluent layer of HeLa cells. A prerequisite of this activity is that the pathogen binds to the cell surface. YopE also induces a cytotoxic response on mouse macrophages where it influences the ability of the pathogen to resist phagocytosis. Bacterial mutants defective in their ability to express YopE are avirulent after oral or intraperitoneal infection but virulent following intravenous injection. On the basis of these results, we propose a role for YopE in the virulence process of Yersinia.