Hemolymph phenol oxidases in Drosophila melanogaster, Locusta migratoria, and Austropotamobius pallipes.
Hemolymph phenol oxidases in Drosophila melanogaster, Locusta migratoria, and Austropotamobius pallipes.
复制标题
果蝇、飞蝗和 Austropotamobius pallipes 中的血淋巴酚氧化酶。
DOI：
10.1016/s0006-291x(05)81129-5
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发表时间：
1991
影响因子：
3.1
通讯作者：
Seymour,J
中科院分区：
文献类型：
--
作者：
Nappi,AJ;Seymour,J
在从L-酪氨酸生物合成黑色素中的早期酶介导的反应序列涉及芳香族氨基酸前体的初始羟基化（单酚氧化酶活性，MPO）以形成L-多巴（3，4-二羟基苯丙氨酸），以及随后所得二酚的氧化（二酚氧化酶活性，DPO）以形成多巴醌。用高压液相色谱-电化学检测法（HPLC-ED）测定了黑腹果蝇（Drosophilamelanogaster）和东亚飞蝗（Locustamigratoria）三龄幼虫和淡水螯虾（Austropotamobiuspallipes）成虫血液（血淋巴）中的酚氧化酶活性。这些结果表明，在每个物种的MPO和DPO可以很容易地检测到不使用外源性激活剂。
The early enzyme-mediated reaction sequence in the biosynthesis of melanin from L-tyrosine involves an initial hydroxylation (monophenol oxidase activity, MPO) of the aromatic amino acid precursor to form L-dopa (3,4-dihydroxyphenylalanine), and the ensuing oxidation (diphenol oxidase activity, DPO) of the resultant diphenol to form dopaquinone. By means of high pressure liquid chromatography with electrochemical detection (HPLC-ED) both phenol oxidase activities were observed in the blood (hemolymph) of two species of insect, third-stage larvae ofDrosophila melanogasterand adultLocusta migratoria, and in an adult fresh-water crayfish,Austropotamobius pallipes. These results establish that in each species MPO and DPO can be detected readily without the use of exogenous activators.