Gliogenic radial glial cells show heterogeneity in the developing mouse spinal cord
Gliogenic radial glial cells show heterogeneity in the developing mouse spinal cord
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DOI：
10.1159/000088452
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发表时间：
2005-01-01
影响因子：
2.9
通讯作者：
Ikenaka, K
中科院分区：
文献类型：
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作者：
Ogawa, Y;Takebayashi, H;Ikenaka, K
哺乳动物胚胎的中枢神经系统是根据沿前后轴和/或背腹轴的区域特异性转录因子的表达来组织的。例如，胚胎脊髓的背室区（VZ）表达Pax3和Pax7，腹侧VZ表达Pax6，更腹侧的VZ表达Nkx2.2。位于VZ的神经元前体的特性是由这些转录因子的特征表达模式决定的，从而导致不同类别神经元的产生。最近的研究表明，在中枢神经系统发育过程中，放射状胶质细胞除了产生胶质细胞外，还产生神经元。因此，神经元前体细胞的多样性可能依赖于放射状胶质细胞的多样性。为了验证这一假说，我们分析了小鼠胚胎脊髓中放射状胶质细胞标记物和转录因子的表达。我们发现放射状胶质细胞确实表达域特异性转录因子。此外，它们在星形胶质细胞特异性谷氨酸转运蛋白的表达上也存在差异。星形胶质细胞特异性谷氨酸转运体在腹侧放射状胶质细胞中强表达的区域与pax6表达域密切相关，弱表达区域与nkx2.2表达域对应。此外，背侧径向纤维表达ephrin-B1。因此，不同类型的放射状胶质细胞存在于E12.5转录因子表达定义的不同区域中。我们还表明，这种多样性持续到放射状胶质细胞的胶质形成阶段。这就提出了一种想法，即小鼠脊髓中沿背腹轴不同区域产生的星形胶质细胞具有不同的特征。版权所有(c) 2005 S. Karger AG，巴塞尔。
The central nervous system of the mammalian embryo is organized according to the expression of region-specific transcription factors along the anteroposterior and/or the dorsoventral axis. For example, the dorsal ventricular zone (VZ) of the embryonic spinal cord expresses Pax3 and Pax7, the ventral VZ expresses Pax6, and the more ventral VZ expresses Nkx2.2. Properties of neuronal precursors located in the VZ are determined by the characteristic expression patterns of these transcription factors, leading to the generation of distinct classes of neurons. Recent studies demonstrated that radial glial cells produce neurons in addition to glia during central nervous system development. Thus, neuronal precursor diversity may be dependent upon the diversity of radial glial cells. To investigate this hypothesis, we analyzed the expression of radial glial cell markers and transcription factors in the mouse embryonic spinal cord. We show that radial glial cells indeed express domain-specific transcription factor. Moreover, they varied in expression of the astrocyte-specific glutamate transporter. The region where the astrocyte-specific glutamate transporter is strongly expressed in the ventral radial glial cells is closely related to the Pax6-expressing domain, and the weakly expressing region corresponding to the Nkx2.2-expressing domain. Furthermore, dorsal radial fibers expressed ephrin-B1. Thus, different types of radial glial cells exist in different domains defined by the transcription factor expression at E12.5. We also show that this diversity continues to the gliogenic stage of radial glial cells. This raises the idea that astrocytes generated from different domains along the dorsoventral axis in the mouse spinal cord have distinct characteristics. Copyright (c) 2005 S. Karger AG, Basel.