The characterization of catecholamine stimulation of glucose transport by rat adipocytes and isolated plasma membranes.
The characterization of catecholamine stimulation of glucose transport by rat adipocytes and isolated plasma membranes.
复制标题
儿茶酚胺刺激大鼠脂肪细胞和分离质膜葡萄糖转运的表征。
作者：
C. Ludvigsen;L. Jarett;J. McDonald
这些研究旨在解决儿茶酚胺刺激葡萄糖转运本身而不仅仅是脂肪细胞中葡萄糖利用的能力的争议。使用双标记（3-O-[3H]甲基葡萄糖和l-[14C]葡萄糖）程序进行葡萄糖转运测定。将完整脂肪细胞与肾上腺素预孵育 15 分钟，使 5 × 10−8 m 处的特异性 3-O-甲基葡萄糖转运增加 15%，而在 2.5 × 10−7 m 处达到对照水平两倍的最大刺激。 10−5 m 普萘洛尔抑制了 95% 的葡萄糖转运刺激。异丙肾上腺素 (10−7 m) 也刺激 3-O-甲基葡萄糖转运，这种效应被 10−5 m 普萘洛尔完全阻断。最大剂量（5 × 10−6 m）的去氧肾上腺素产生边缘（20-30%）运输刺激，而酚妥拉明仅部分（50%）抑制该运输刺激。比较了肾上腺素和胰岛素对 3-O-甲基葡萄糖转运的刺激，肾上腺素对转运的最大刺激...
These studies were designed to resolve the controversy over the ability of catecholamines to stimulate glucose transport per se and not just glucose utilization in adipocytes. Glucose transport assays were performed using a dual label (3- O-[3H]methylglucose and l-[14C]glucose) procedure. Fifteenminute preincubations of intact adipocytes with epinephrine produced a 15% increase in specific 3-O-methylglucose transport at 5 × 10−8 m, while a maximal stimulation of twice the control level was reached at 2.5 × 10−7 m. This stimulation of glucose transport was inhibited 95% by 10−5 m propranolol. Isoproterenol (10−7 m) also stimulated 3-O-methylglucose transport, and this effect was completely blocked by 10−5 m propranolol. Phenylephrine at maximal doses (5 × 10−6 m) produced a marginal (20–30%) stimulation of transport which was only partially (50%) inhibited by phentolamine. Epinephrine and insulin stimulation of 3-O-methylglucose transport were compared, and the maximal stimulation of transport by epinephri...