Telomerase activation induces elongation of the telomeric single-stranded overhang, but does not prevent chromosome aberrations in human vascular endothelial cells
Telomerase activation induces elongation of the telomeric single-stranded overhang, but does not prevent chromosome aberrations in human vascular endothelial cells
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DOI：
10.1016/j.bbrc.2006.12.112
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发表时间：
2007-02-23
影响因子：
3.1
通讯作者：
Tahara, Hidetoshi
中科院分区：
文献类型：
--
作者：
Anno, Kumiko;Hayashi, Akane;Tahara, Hidetoshi
在人脐带静脉 (HUVEC) 内皮细胞中经常观察到染色体畸变，例如 13 号染色体缺失。最近的一项研究表明，作为染色体维持的重要结构，端粒单链 3' 突出端（G 尾）的长度比端粒 T 环形成中的净端粒长度更为重要。在这里，我们使用正常和 hTERT 转导的 HUVEC 中的 G 尾端粒 HPA 检查了 G 尾长度。我们发现 HUVEC 中 hTERT 的强制表达诱导 G 尾以及总端粒长度延长。 G尾长度与端粒总长度密切相关。然而，hTERT的引入并不能阻止染色体畸变，例如13号染色体丢失。与年轻的正常HUVEC一样，在hTERT-HUVEC中观察到正常特征，例如形态、vWF上调和管形成。这些结果表明 HUVEC 中的染色体畸变与端粒 G 尾和总端粒磨损无关。 (c) 2006 Elsevier Inc. 保留所有权利。
Chromosome aberrations such as loss of chromosome 13 were frequently observed in human endothelial cells from umbilical cord veins (HUVEC). A recent study showed that the length of telomeric single-stranded 3'-overhangs (G-tails) is more important as an essential structure for chromosome maintenance than the net telomere length in telomere t-loop formation. Here, we have examined G-tail length using G-tail telomere HPA in normal and hTERT-transduced HUVECs. We found that forced expression of hTERT in HUVEC induced G-tail as well as total telomere length elongation. G-tail length was well correlated with total telomere length. However, hTERT introduction did not prevent chromosome aberrations such as loss of chromosome 13. Normal characteristics such as morphology, up-regulation of vWF, and tube formation were observed in hTERT-HUVEC as in young normal HUVEC. These results show that chromosome aberrations in HUVEC are independent of telomere G-tail and total telomere attrition. (c) 2006 Elsevier Inc. All rights reserved.