CROSS-REACTIVITY OF SELECTED COMPOUNDS IN THE ABBOTT TDX CANNABINOID ASSAY
CROSS-REACTIVITY OF SELECTED COMPOUNDS IN THE ABBOTT TDX CANNABINOID ASSAY
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DOI：
10.1093/jat/14.5.277
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发表时间：
1990-09-01
影响因子：
2.5
通讯作者：
ELSOHLY, HN
中科院分区：
文献类型：
--
作者：
ELSOHLY, MA;JONES, AB;ELSOHLY, HN
免疫分析程序，包括酶免疫分析和放射免疫分析，继续被广泛用于通过分析尿样中的11-nor-.DELTA.9-tetrahydrocannabinol-9-carboxylic酸(9-四氢大麻酚的主要尿代谢物--11-或9-四氢大麻酚)来筛查最近使用大麻的样品。使用市售免疫分析试剂，对用于雅培公司S TDX大麻素荧光偏振免疫分析的抗血清的交叉反应性进行了评价。这种交叉反应是针对大麻中的一组大麻素和非大麻素酚成分、一些大麻素代谢物和出现在正常尿样中的其他药物进行评估的。一般而言，抗血清对11-nor-.DELTA.8-tetrahydrocannabinol-9-carboxylic酸、其葡萄糖醛酸苷和相应的β8-异构体具有同样的反应，该异构体是在1990年1月之前的分析标准和对照中使用的酸性部分。9-和8-THC的羟化衍生物降低了与抗血清的结合，其他大麻素对抗体的结合潜力有限。对于非大麻类成分，在所评估的最高浓度(40 mg/L)下没有观察到结合。
Immunoassay procedures, both enzyme immunoassay and radioimmunoassay, continue to be widely used to screen samples for recent marijuana use by analyzing the urine samples for 11-nor-.DELTA.9-tetrahydrocannabinol-9-carboxylic acid (11-nor-.DELTA.9-THC-9-COOH) (the major urinary metabolite of .DELTA.9-tetrahydrocannabinol [.DELTA.9-THC]). Using commercially available immunoassay reagents, the cross-reactivity of the antiserum utilized in Abbott''s TDx cannabinoid assay (a fluorescence polarization immunoassay) was evaluated. This cross-reactivity was evaluated against a group of cannabinoids and noncannabinoid phenolic constituents of Cannabis, some cannabinoid metabolites, and other agents that appear in normal urine samples. In general, the antiserum was equally reactive toward 11-nor-.DELTA.8-tetrahydrocannabinol-9-carboxylic acid, its glucuronide, and the corresponding .DELTA.8-isomer, which was the acid moiety utilized in standards and controls of the assay prior to January, 1990. Reduced binding to the antiserum was observed with hydroxylated derivatives of .DELTA.9- and .DELTA.8-THC, and the other cannabinoids, in general, exhibited limited binding potentials towards the antibody. For the noncannabinoid constituents, no binding was observed at the highest concentrations evaluated (40 mg/L).